DOI: 10.1128/CVI.00536-12
Article Figures & Data
Figures
- Fig 1
MAb binding patterns for purified GXM (A) and discordant patient specimens (B). Refer to Materials and Methods for a detailed description of the anti-GXM EIA. MAb, monoclonal antibody; GXM, glucuronoxylomannan.
Tables
- Table 1
Qualitative test results
IMMY test result No. of samples with Meridian EIA result CSF Serum Positive Negative Positive Negative IMMY LFA results Positive 15 1 41 21 Negative 0 395 0 527 IMMY EIA results Positive 15 2 41 19 Negative 0 394 0 529 - Table 2
Qualitative assay agreement
Comparison and agreement % Agreement Kappa value (95% CI) Meridian EIA vs IMMY LFA 97.8 0.82 (0.75–0.9) Positive agreement 71.8 Negative agreement 97.7 Meridian EIA vs IMMY EIA 98.0 0.84 (0.77–0.91) Positive agreement 73.7 Negative agreement 97.9 IMMY LFA vs IMMY EIA 99.7 0.98 (0.96–1.00) Positive agreement 97.4 Negative agreement 99.9 - Table 3
Discrepant specimen resultsa
Specimen ID no. Source IMMY LFA result LFA titer IMMY EIA result EIA titer Meridian EIA result IMMY latex test result Latex test titer GXM serotype 058 Serum POS <1:5 POS 1:5 NEG QNS NA NA 804 Serum POS 1:44 POS 1:40 NEG QNS NA NA 122 Serum POS 1:35 POS 1:40 NEG QNS NA NA 104 Serum POS 1:14 POS 1:5 NEG QNS NA NA 953 Serum POS 1:17 POS 1:5 NEG QNS NA NA 207 Serum POS 1:59 POS 1:80 NEG QNS NA NA 749 Serum POS <1:5 POS 1:5 NEG QNS NA NA 894 Serum POS <1:5 POS 1:5 NEG QNS NA NA 046 Serum POS <1:5 POS 1:5 NEG QNS NA NA 520 Serum POS 1:14 POS 1:5 NEG QNS NA NA 806 Serum POS QNS POS 1:20 NEG QNS NA NA 309 Serum NEG NA POS 1:5 NEG QNS NA NA 777 CSF POS QNS NEG NA NEG NEG NA NA 287 Serum POS 1:12 POS 1:5 NEG POS QNS C-like 028 Serum POS <1:5 POS 1:5 NEG POS 1:2 INDETERM 603 Serum POS 1:86 POS 1:40 INDETERM POS 1:32 C-like 963 CSF POS 1:10 POS 1:5 NEG NEG NA INDETERM 050 Serum POS 1:27 POS 1:10 INDETERM POS 1:8 INCONb 497 Serum POS 1:105 POS 1:320 NEG POS 1:32 C-like 378 Serum POS <1:5 POS 1:5 NEG POS <1.2 INDETERM 161 Serum NEG NA POS 1:5 NEG NEG NA INDETERM 780 Serum POS <1:5 POS 1:10 NEG POS <1:2 INDETERM 785 Serum POS 1:62 POS 1:320 NEG POS 1:128 C-like ↵a Abbreviations: POS, positive; NEG, negative; NA, not applicable; QNS, quantity of the specimen was not sufficient for repeat testing; INDETERM, indeterminate; INCON, inconclusive; GXM, glucuronoxylomannan.
↵b MAb reactivity was not typical of patterns observed with GXM from the four major serotypes.
Large-Scale Evaluation of the Immuno-Mycologics Lateral Flow and Enzyme-Linked Immunoassays for Detection of Cryptococcal Antigen in Serum and Cerebrospinal Fluid
