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Clinical Immunology

Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion

Suzanne J. C. Verhaegh, Kim Stol, Corné P. de Vogel, Kristian Riesbeck, Eric R. Lafontaine, Timothy F. Murphy, Alex van Belkum, Peter W. M. Hermans, John P. Hays
Suzanne J. C. Verhaegh
aDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
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Kim Stol
bDepartment of Pediatrics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
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Corné P. de Vogel
aDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
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Kristian Riesbeck
cMedical Microbiology, Department of Laboratory Medicine Malmö, Skåne University Hospital, Lund University, Malmö, Sweden
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Eric R. Lafontaine
dDepartment of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
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Timothy F. Murphy
eDepartment of Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA
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Alex van Belkum
aDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
fLa Balme Microbiology Unit, bioMérieux, La Balme les Grottes, France
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Peter W. M. Hermans
bDepartment of Pediatrics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
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John P. Hays
aDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
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DOI: 10.1128/CVI.05630-11
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ABSTRACT

A prospective clinical cohort study was established to investigate the humoral immune response in middle ear fluids (MEF) and serum against bacterial surface proteins in children suffering from recurrent acute otitis media (rAOM) and chronic otitis media with effusion (COME), using Luminex xMAP technology. The association between the humoral immune response and the presence of Moraxella catarrhalis and Streptococcus pneumoniae in the nasopharynx and middle ear was also studied. The levels of antigen-specific IgG, IgA, and IgM showed extensive interindividual variation. No significant differences in anti-M. catarrhalis and anti-S. pneumoniae serum and MEF median fluorescence intensity (MFI) values (anti-M. catarrhalis and antipneumococcal IgG levels) were observed between the rAOM or COME groups for all antigens tested. No significant differences were observed for M. catarrhalis and S. pneumoniae colonization and serum IgG levels against the Moraxella and pneumococcal antigens. Similar to the antibody response in serum, no significant differences in IgG, IgA, and IgM levels in MEF were observed for all M. catarrhalis and S. pneumoniae antigens between OM M. catarrhalis- or S. pneumoniae-positive and OM M. catarrhalis- or S. pneumonia-negative children suffering from either rAOM or COME. Finally, results indicated a strong correlation between antigen-specific serum and MEF IgG levels. We observed no significant in vivo expressed anti-M. catarrhalis or anti-S. pneumoniae humoral immune responses using a range of putative vaccine candidate proteins. Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME.

  • Copyright © 2012, American Society for Microbiology. All Rights Reserved.
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Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion
Suzanne J. C. Verhaegh, Kim Stol, Corné P. de Vogel, Kristian Riesbeck, Eric R. Lafontaine, Timothy F. Murphy, Alex van Belkum, Peter W. M. Hermans, John P. Hays
Clinical and Vaccine Immunology May 2012, 19 (6) 914-918; DOI: 10.1128/CVI.05630-11

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Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion
Suzanne J. C. Verhaegh, Kim Stol, Corné P. de Vogel, Kristian Riesbeck, Eric R. Lafontaine, Timothy F. Murphy, Alex van Belkum, Peter W. M. Hermans, John P. Hays
Clinical and Vaccine Immunology May 2012, 19 (6) 914-918; DOI: 10.1128/CVI.05630-11
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