Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Clinical and Vaccine Immunology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
Letters to the Editor

Poliovirus Neutralization Test with Poliovirus Pseudovirus To Measure Neutralizing Antibody in Humans

Subhash C. Arya, Nirmala Agarwal
Subhash C. Arya
Sant Parmanand HospitalDelhi, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nirmala Agarwal
Sant Parmanand HospitalDelhi, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/CVI.05568-11
  • Article
  • Info & Metrics
  • PDF
Loading

LETTER

We complement Arita et al. (1) for their development of a safe, simple, and rapid test, employing poliovirus pseudovirus (generating luciferase signals) instead of the wild poliovirus, to measure the poliovirus neutralizing antibody titer in human serum. Apart from the end game of the global poliomyelitis eradication program, poliovirus pseudovirus technology (1) would be advantageous in the surveillance of cohorts of children and adults rendered poliovirus susceptible after therapeutic interventions for malignancy or organ transplants.

Some children and adults under chemotherapy regimens after organ transplants, as well as those infected with human immunodeficiency virus, constitute cohorts lacking poliovirus neutralizing antibodies (3). Cytostatic therapy for acute lymphocytic leukemia in children results in a temporary reduction of specific antibody levels. Both the reduced and standard chemotherapy regimens for acute childhood lymphoblastic leukemia in Utrecht, Netherlands, were accompanied by decreased antibody levels against vaccine-preventable diseases (5).

During future fine-tuning of the threshold values for protective antibody against the three poliovirus serotypes, it would be desirable to express poliovirus neutralizing titer obtained with pseudovirus technology (1) in international units rather than an arbitrary dilution figure. Comparison of inter- and intralaboratory serologic data would be more explicit using international units. Rather than an arbitrary dilution figure, the antibody content would be more explicit in international units. Serologic data on representative post-oral-poliovirus-vaccine sera from Germany were expressed in such units (2). Furthermore, protective neutralizing antibody titers for three polioviruses in 1,133 individuals older than 2 years in mainland Portugal were also expressed in international units (4).

ACKNOWLEDGMENTS

The authors declare no conflict of interest and that no ethical clearance was required and no financial support provided.

FOOTNOTES

  • For the author reply, see doi:10.1128/CVI.05698-11.

  • Copyright © 2012, American Society for Microbiology. All Rights Reserved.

REFERENCES

  1. 1.↵
    1. Arita M,
    2. Iwai M,
    3. Wakita T,
    4. Shimizu H
    . 2011. Development of a poliovirus neutralization test with poliovirus pseudovirus for measurement of neutralizing antibody titer in human serum. Clin. Vaccine Immunol. 18:1889–1894.
    OpenUrlAbstract/FREE Full Text
  2. 2.↵
    1. Diedrich S,
    2. Claus H,
    3. Schreier E
    . 2002. Immunity against poliomyelitis in Germany: determination of cut-off values in international units. BMC Infect. Dis. 2:2.
    OpenUrlCrossRefPubMed
  3. 3.↵
    1. Machado CM
    . 2004. Re-immunization after bone marrow transplantation—current recommendations and perspectives. Braz. J. Med. Biol. Res. 37:151–158.
    OpenUrlPubMed
  4. 4.↵
    1. Pires de Miranda M,
    2. Carmo Gomes M,
    3. Rebelo de Andrade H
    . 2007. Seroprevalence of antibodies to poliovirus in individuals living in Portugal, 2002. Euro Surveill. 12:E7–E8.
    OpenUrlPubMed
  5. 5.↵
    1. van Tilburg CM,
    2. et al
    . 25 July 2011. Impact of treatment reduction for childhood acute lymphoblastic leukemia on serum immunoglobulin and antibodies against vaccine-preventable diseases. Pediatr. Blood Cancer [Epub ahead of print]. doi:10.1002/pbc.23258.
PreviousNext
Back to top
Download PDF
Citation Tools
Poliovirus Neutralization Test with Poliovirus Pseudovirus To Measure Neutralizing Antibody in Humans
Subhash C. Arya, Nirmala Agarwal
Clinical and Vaccine Immunology Feb 2012, 19 (3) 458; DOI: 10.1128/CVI.05568-11

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Clinical and Vaccine Immunology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Poliovirus Neutralization Test with Poliovirus Pseudovirus To Measure Neutralizing Antibody in Humans
(Your Name) has forwarded a page to you from Clinical and Vaccine Immunology
(Your Name) thought you would be interested in this article in Clinical and Vaccine Immunology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Poliovirus Neutralization Test with Poliovirus Pseudovirus To Measure Neutralizing Antibody in Humans
Subhash C. Arya, Nirmala Agarwal
Clinical and Vaccine Immunology Feb 2012, 19 (3) 458; DOI: 10.1128/CVI.05568-11
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • LETTER
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About CVI
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1556-6811; Online ISSN: 1556-679X