Article Figures & Data
Tables
- Table 1
Comparison of three cryptococcal antigen assays to the latex agglutination test using serum specimens (n = 634)a
Assay and result No. of samples with the following Meridian LA test result: Sensitivity (%) (95% CI) Specificity (%) (95% CI) Agreement (%) (95% CI) Kappa Positive Negative Premier EIA Positive 5 0 55.6 (26.6, 81.2) 100 (99.3, 100) 99.4 (98.3, 99.8) 0.71 Negative 4 625 Alpha CrAg EIA Positive 9 2b 100 (65.5, 100) 99.7 (98.8, 99.9) 99.7 (98.8, 99.9) 0.90 Negative 0 623 CrAg LFA Positive 9 1c 100 (65.5, 100) 99.8 (99.0, 100) 99.8 (99.0, 99.9) 0.95 Negative 0 624 ↵a LA, latex agglutination; EIA, enzyme immunoassay; LFA, lateral flow assay; 95% CI, 95% confidence interval.
↵b One of these two samples showed 1+ reactivity by the Meridian latex agglutination assay upon screening but was interpreted as negative according to the package insert's requirement for 2+ reactivity.
↵c This sample showed 1+ reactivity by the Meridian latex agglutination assay upon screening but was interpreted as negative according the package insert's requirement for 2+ reactivity.
- Table 2
Comparison of four cryptococcal antigen assays to a consensus of the test panel using serum specimens (n = 633)a
Assay and result No. of samples with consensus of test panel resultsb Sensitivity (%) (95% CI) Specificity (%) (95% CI) Agreement (%) (95% CI) Kappa Positive Negative Meridian LA Positive 9 0 100 (65.5, 100) 100 (99.3, 100) 100 (99.3, 100) 1.0 Negative 0 624 Premier EIA Positive 5 0 55.6 (26.6, 81.2) 100 (99.3, 100) 99.4 (98.3, 99.8) 0.71 Negative 4 624 Alpha CrAg EIA Positive 9 1 100 (65.5, 100) 99.8 (99.0, 99.9) 99.8 (99.0, 99.9) 0.95 Negative 0 623 CrAg LFA Positive 9 0 100 (65.5, 100) 100 (99.3, 100) 100 (99.3, 100) 1.0 Negative 0 624 ↵a LA, latex agglutination; EIA, enzyme immunoassay; LFA, lateral flow assay; 95% CI, 95% confidence interval.
↵b A consensus of the test panel results was defined as at least 3 of 4 cryptococcal antigen results being in agreement. There was one sample that did not yield a consensus result; therefore, only 633 sera were included in this analysis.
