Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Clinical and Vaccine Immunology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
Diagnostic Laboratory Immunology

Comparison of Four Assays for the Detection of Cryptococcal Antigen

M. J. Binnicker, D. J. Jespersen, J. E. Bestrom, L. O. Rollins
M. J. Binnicker
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. J. Jespersen
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. E. Bestrom
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
L. O. Rollins
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/CVI.00446-12
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Tables

  • Table 1

    Comparison of three cryptococcal antigen assays to the latex agglutination test using serum specimens (n = 634)a

    Assay and resultNo. of samples with the following Meridian LA test result:Sensitivity (%) (95% CI)Specificity (%) (95% CI)Agreement (%) (95% CI)Kappa
    PositiveNegative
    Premier EIA
        Positive5055.6 (26.6, 81.2)100 (99.3, 100)99.4 (98.3, 99.8)0.71
        Negative4625
    Alpha CrAg EIA
        Positive92b100 (65.5, 100)99.7 (98.8, 99.9)99.7 (98.8, 99.9)0.90
        Negative0623
    CrAg LFA
        Positive91c100 (65.5, 100)99.8 (99.0, 100)99.8 (99.0, 99.9)0.95
        Negative0624
    • ↵a LA, latex agglutination; EIA, enzyme immunoassay; LFA, lateral flow assay; 95% CI, 95% confidence interval.

    • ↵b One of these two samples showed 1+ reactivity by the Meridian latex agglutination assay upon screening but was interpreted as negative according to the package insert's requirement for 2+ reactivity.

    • ↵c This sample showed 1+ reactivity by the Meridian latex agglutination assay upon screening but was interpreted as negative according the package insert's requirement for 2+ reactivity.

  • Table 2

    Comparison of four cryptococcal antigen assays to a consensus of the test panel using serum specimens (n = 633)a

    Assay and resultNo. of samples with consensus of test panel resultsbSensitivity (%) (95% CI)Specificity (%) (95% CI)Agreement (%) (95% CI)Kappa
    PositiveNegative
    Meridian LA
        Positive90100 (65.5, 100)100 (99.3, 100)100 (99.3, 100)1.0
        Negative0624
    Premier EIA
        Positive5055.6 (26.6, 81.2)100 (99.3, 100)99.4 (98.3, 99.8)0.71
        Negative4624
    Alpha CrAg EIA
        Positive91100 (65.5, 100)99.8 (99.0, 99.9)99.8 (99.0, 99.9)0.95
        Negative0623
    CrAg LFA
        Positive90100 (65.5, 100)100 (99.3, 100)100 (99.3, 100)1.0
        Negative0624
    • ↵a LA, latex agglutination; EIA, enzyme immunoassay; LFA, lateral flow assay; 95% CI, 95% confidence interval.

    • ↵b A consensus of the test panel results was defined as at least 3 of 4 cryptococcal antigen results being in agreement. There was one sample that did not yield a consensus result; therefore, only 633 sera were included in this analysis.

  • Table 3

    Correlation of reciprocal endpoint titers for cryptococcal antigen-positive serum samples by the latex agglutination test and a lateral flow assay

    Serum sampleReciprocal endpoint titer bya:
    LACrAg LFA
    1256160
    2810
    31680
    41,0242,560
    5Negativeb10
    6820
    7210
    8410
    964160
    10820
    • ↵a LA, latex agglutination; LFA, lateral flow assay.

    • ↵b This sample showed 1+ reactivity by the Meridian latex agglutination assay upon screening but was interpreted as negative according to the package insert's requirement for 2+ reactivity.

PreviousNext
Back to top
Download PDF
Citation Tools
Comparison of Four Assays for the Detection of Cryptococcal Antigen
M. J. Binnicker, D. J. Jespersen, J. E. Bestrom, L. O. Rollins
Clinical and Vaccine Immunology Nov 2012, 19 (12) 1988-1990; DOI: 10.1128/CVI.00446-12

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Clinical and Vaccine Immunology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Comparison of Four Assays for the Detection of Cryptococcal Antigen
(Your Name) has forwarded a page to you from Clinical and Vaccine Immunology
(Your Name) thought you would be interested in this article in Clinical and Vaccine Immunology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Comparison of Four Assays for the Detection of Cryptococcal Antigen
M. J. Binnicker, D. J. Jespersen, J. E. Bestrom, L. O. Rollins
Clinical and Vaccine Immunology Nov 2012, 19 (12) 1988-1990; DOI: 10.1128/CVI.00446-12
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • TEXT
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About CVI
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1556-6811; Online ISSN: 1556-679X