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Diagnostic Laboratory Immunology

Evaluation of Gamma Interferon and Antibody Tuberculosis Tests in Alpacas

Shelley Rhodes, Tom Holder, Derek Clifford, Ian Dexter, Jacky Brewer, Noel Smith, Laura Waring, Tim Crawshaw, Steve Gillgan, Konstantin Lyashchenko, John Lawrence, John Clarke, Ricardo de la Rua-Domenech, Martin Vordermeier
Shelley Rhodes
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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Tom Holder
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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Derek Clifford
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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Ian Dexter
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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Jacky Brewer
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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Noel Smith
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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Laura Waring
bAHVLA—Langford, Bristol, United Kingdom
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Tim Crawshaw
cAHVLA—Starcross, Staplake Mount, Starcross, Exeter, Devon, United Kingdom
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Steve Gillgan
dAHVLA—Worcester, United Kingdom
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Konstantin Lyashchenko
eChembio Diagnostic Systems, Inc., Medford, New York, USA
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John Lawrence
fIdexx Laboratories, Inc., Westbrook, Maine, USA
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John Clarke
gEnfer Scientific, Unit 7, Newhall, Naas, Kildare, Ireland
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Ricardo de la Rua-Domenech
hAHVLA-TB Programme, Defra, London, United Kingdom
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Martin Vordermeier
aAnimal Health and Veterinary Laboratories Agency (AHVLA)—Weybridge, Addlestone, Surrey, United Kingdom
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DOI: 10.1128/CVI.00405-12
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Article Figures & Data

Tables

  • Table 1

    Observed IFN-γ test sensitivity and specificity values

    Test resultaVL alpacas (n = 55)TB-free alpacas (n = 257)
    n/55% sensitivity95% CIn/257% specificity95% CI
    PPD+3563.650.9–76.32889.185.3–92.9
    EC+2749.135.9–62.32689.986.2–93.6
    EC+ PPD−814.55.2–23.82191.888.4–95.2
    PPD+ EC+1934.622.0–47.2598.196.4–99.8
    PPD+ or EC+4480.069.4–90.64980.976.1–85.7
    • ↵a PPD, PPDB − PPDA; EC, ESAT6-CFP10 peptide cocktail.

  • Table 2

    Increasing the IFN-γ test specificity values

    Test resultaVL alpacas (n = 55)TB-free alpacas (n = 257)
    n/55% sensitivity95% CIn/257% specificity95% CI
    PPD+1629.117.1–41.1896.994.8–99.0
    EC+1629.117.1–41.1896.994.8–99.0
    EC+ PPD−712.73.9–21.5896.994.8–99.0
    PPD+ EC+916.46.6–26.10100.098.6–100
    PPD+ or EC+2341.828.8–54.81693.890.9–96.7
    • ↵a PPD, PPDB − PPDA; EC, ESAT6-CFP10 peptide cocktail.

  • Table 3

    Antibody test sensitivity and specificity values

    TestVL alpacasTB-free alpacas
    n/total% sensitivity95% CIn/total% specificity95% CI
    Stat-Pak35/5267.354.5–80.88/30697.495.6–99.2
    DPP30/5257.744.3–71.110/30696.794.1–98.4
    Idexx36/5269.256.7–81.78/30697.495.6–99.2
    Enferplex32/4866.753.4–80.08/25796.994.8–99.0
  • Table 4

    IFN-γ and antibody test combinations and sensitivity and specificity valuesa

    Test combinationVL alpacas (n = 48)TB-free alpacas (n = 257)
    n/48% sensitivity95% CIn/257% specificity95% CI
    IFN-γ + Stat-Pak4593.887.0–1005279.274.2–84.2
    IFN-γ + Idexx4491.783.9–99.55578.673.6–83.6
    IFN-γ + DPP4491.783.9–99.55877.472.3–82.5
    IFN-γ + Enferplex4593.887–1005877.472.3–82.5
    IFN-γ + Stat-Pak + Idexx4810092.6–1005777.872.7–82.9
    IFN-γ + Stat-Pak + DPP4810092.6–1006076.771.5–81.9
    IFN-γ + Stat-Pak + Enferplex4797.993.8–1006076.771.5–81.9
    IFN-γ + Idexx + DPP4593.887.0–1006275.970.0–81.2
    IFN-γ + Idexx + Enferplex4491.783.9–99.56275.970.0–81.2
    IFN-γ + Enferplex + DPP4797.993.8–1006574.769.4–80
    • ↵a IFN-γ positive, PPDB-PPDA > 0.1 or EC-nil > 0.1.

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Evaluation of Gamma Interferon and Antibody Tuberculosis Tests in Alpacas
Shelley Rhodes, Tom Holder, Derek Clifford, Ian Dexter, Jacky Brewer, Noel Smith, Laura Waring, Tim Crawshaw, Steve Gillgan, Konstantin Lyashchenko, John Lawrence, John Clarke, Ricardo de la Rua-Domenech, Martin Vordermeier
Clinical and Vaccine Immunology Sep 2012, 19 (10) 1677-1683; DOI: 10.1128/CVI.00405-12

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Evaluation of Gamma Interferon and Antibody Tuberculosis Tests in Alpacas
Shelley Rhodes, Tom Holder, Derek Clifford, Ian Dexter, Jacky Brewer, Noel Smith, Laura Waring, Tim Crawshaw, Steve Gillgan, Konstantin Lyashchenko, John Lawrence, John Clarke, Ricardo de la Rua-Domenech, Martin Vordermeier
Clinical and Vaccine Immunology Sep 2012, 19 (10) 1677-1683; DOI: 10.1128/CVI.00405-12
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    • ABSTRACT
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