Article Figures & Data
Figures
- Fig. 1.
Chromatogram of the FPLC protein complexes isolated from a mixture of cyathostomin third-stage larvae. AU, arbitrary units.
- Fig. 2.
ROC analysis of the results achieved by using the FPLC protein complexes isolated from a mixture of cyathostomin third-stage larvae and sera from infected and uninfected horses.
- Fig. 3.
Kinetics of cyathostomin egg output in naturally infected weanlings. Values along the y axis represent numbers of cythostomin eggs per gram of feces (EPG). Data represent the mean values plus 2 standard deviations.
- Fig. 4.
Kinetics of IgG(T) antibodies against antigenic peaks P1 (A), P2 (B), and P3 (C) isolated from L3 cyathostomin excretory/secretory antigens by means of an FPLC system. Values along the y axis represent optical density at 492 nm. Data represent the mean values plus 2 standard deviations.
Tables
- Table 1.
Gel filtration chromatography protocol
Step Vol (ml) Description 1 0.00 Collection of 4-ml fractions during entire run 2 0.00 Lamp (UV detector) turned on 3 0.00 Zero baseline 4 0.00 Isocratic flow 5 5.00 Load/inject 2.5-ml sample 6 7.5 Isocratic flow 7 600 End of protocol - Table 2.
Analysis of the liquid chromatography (FPLC) protein complexes isolated from a mixture of cyathostomin third-stage larvaea
Antigen peak and size (kDa) Cutoff value AUC P S (%) SP (%) PLR NLR P1 (51) 0.4850 0.982 0.001 92 98 46 0.08 P2 (29) 0.4965 0.985 0.001 92 94 15 0.08 P3 (15) 0.4815 0.982 0.001 92 91 10 0.09 ↵a The cutoff point for each antigen was established on the basis of the highest values achieved for the AUC (maximum 1), S (sensitivity, 0 to 100), SP (specificity, 0 to 100), PLR (positive-likelihood ratio; elevated numbers are expected), and NLR (negative-likelihood ratio; low values are expected).
