Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Clinical and Vaccine Immunology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
CLINICAL LABORATORY IMMUNOLOGY

Evaluation of a Diagnostic Algorithm for Acute Q Fever in an Outbreak Setting

Mischa M. Jager, Gezina Weers-Pothoff, Mirjam H. A. Hermans, Jamie C. E. Meekelenkamp, Jeroen J. A. Schellekens, Nicole H. M. Renders, Alexander C. A. P. Leenders, Peter M. Schneeberger, Peter C. Wever
Mischa M. Jager
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, NetherlandsDepartment of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gezina Weers-Pothoff
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mirjam H. A. Hermans
Molecular Diagnostics, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jamie C. E. Meekelenkamp
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeroen J. A. Schellekens
Molecular Diagnostics, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicole H. M. Renders
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alexander C. A. P. Leenders
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter M. Schneeberger
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter C. Wever
Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: p.wever@jbz.nl
DOI: 10.1128/CVI.00009-11
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Diagnostic algorithm for acute Q fever in an outbreak setting introduced 1 May 2009 in the Jeroen Bosch Hospital, 's-Hertogenbosch, Netherlands. Abbreviations: ELISA MII-screen, enzyme-linked immunosorbent assay for immunoglobulin M antibodies to Coxiella burnetii phase II antigens; IFA, immunofluorescence assay; IgM-II antibodies, immunoglobulin M antibodies to C. burnetii phase II antigens; IgG-II antibodies, immunoglobulin G antibodies to C. burnetii phase II antigens; IgG-I antibodies, immunoglobulin G antibodies to C. burnetii phase I antigens.

Tables

  • Figures
  • Table 1.

    Outcomes of the diagnostic algorithm for acute Q fever in an outbreak setting and definitions

    Outcome in the diagnostic algorithmDefinition
    Acute Q feverPresence of IgM and IgG antibodies to phase II antigens with or without IgM and IgG antibodies to phase I antigens or PCR positivity
    Past resolved Q feverPresence of IgG antibodies to phase II antigens with or without IgG antibodies to phase I antigens in the absence of IgM antibodies
    No acute Q feverAbsence of a serologic response in serum samples obtained >14 days after onset of disease
    Cross-reactivityPresence of false-positive IgM antibodies to phase II antigens detected by ELISA but not confirmed by additional tests in first or follow-up serum samples
    Inconclusive outcomeNoncompliance with the request to refer a follow-up serum sample
    Inconsistency with the diagnostic algorithmNonadherence to the flowchart of the diagnostic algorithm
  • Table 2.

    Outcome of acute Q fever diagnostics according to a diagnostic algorithm in 825 patients referred from 15 to 31 May 2009

    StatusNo. of samples with definitive outcomeRelative outcome overall (%)
    In first serum sampleIn follow-up serum sampleOverall
    Acute Q fever1905524529.7
    Past resolved Q fever2130.4
    No acute Q fever11612924529.7
    Cross-reactivity0440.5
    Inconclusive outcome299230136.5
    Inconsistency with the diagnostic algorithm
        Acute Q fever7411
        No acute Q fever549
        Cross-reactivity022
        Inconclusive outcome505
        Total with inconsistent result1710273.3
PreviousNext
Back to top
Download PDF
Citation Tools
Evaluation of a Diagnostic Algorithm for Acute Q Fever in an Outbreak Setting
Mischa M. Jager, Gezina Weers-Pothoff, Mirjam H. A. Hermans, Jamie C. E. Meekelenkamp, Jeroen J. A. Schellekens, Nicole H. M. Renders, Alexander C. A. P. Leenders, Peter M. Schneeberger, Peter C. Wever
Clinical and Vaccine Immunology Jun 2011, 18 (6) 963-968; DOI: 10.1128/CVI.00009-11

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Clinical and Vaccine Immunology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Evaluation of a Diagnostic Algorithm for Acute Q Fever in an Outbreak Setting
(Your Name) has forwarded a page to you from Clinical and Vaccine Immunology
(Your Name) thought you would be interested in this article in Clinical and Vaccine Immunology.
Share
Evaluation of a Diagnostic Algorithm for Acute Q Fever in an Outbreak Setting
Mischa M. Jager, Gezina Weers-Pothoff, Mirjam H. A. Hermans, Jamie C. E. Meekelenkamp, Jeroen J. A. Schellekens, Nicole H. M. Renders, Alexander C. A. P. Leenders, Peter M. Schneeberger, Peter C. Wever
Clinical and Vaccine Immunology Jun 2011, 18 (6) 963-968; DOI: 10.1128/CVI.00009-11
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About CVI
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

Copyright © 2019 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1556-6811; Online ISSN: 1556-679X