Article Figures & Data
Figures
- Fig. 1.
Clinical disease. All dogs were challenged with CIV on study day 35 and monitored daily from day −2 through 13 days postchallenge for clinical signs. One dog in group 1 was euthanized at 9 days postchallenge, and one dog in group 3 died at 8 days postchallenge. The average clinical scores for each treatment group were plotted against time (days after CIV challenge).
- Fig. 2.
Virus shedding. All dogs in groups 1, 3, and 4 were challenged with CIV on study day 35 (day 0 after CIV challenge). Nasal swabs were collected on the day before challenge (day −1) to confirm that the dogs were free of CIV shedding. Nasal virus shedding was monitored in challenged dogs daily for 10 days (days 1 to 10 postchallenge). One dog in group 1 was euthanized at 9 days postchallenge, and one dog in group 3 died at 8 days postchallenge. The average virus titers for each treatment group, expressed as log10 TCID50/ml, were calculated and plotted against time (days after CIV challenge).
- Fig. 3.
Bacterial isolation from nasal swabs and lung washes. All dogs in groups 2, 3, and 4 were challenged with CIV on study day 35 (day 0) and with S. equi subsp. zooepidemicus on study day 38 (day 3 after CIV challenge). Nasal swabs for bacterial isolation were collected on the day before CIV challenge (day −1) and 3, 6, 9, 12, and 14 days postchallenge. At the time of necropsy, lung wash samples were also collected. The results are expressed as the percentage of dogs positive for S. equi subsp. zooepidemicus.
- Fig. 4.
Lung lesions. The gross lung lesion scores are shown. One dog in group 1 was euthanized at 9 days postchallenge, and one dog in group 3 died at 8 days postchallenge. All remaining dogs were euthanized at the end of the study (study day 49). Lung lesions were scored for consolidation, and the total lesion score for each dog was calculated.
- Fig. 5.
Examples of histopathologic lung lesions. Lung sections from a healthy dog (A), from a dog challenged with CIV only (B), from a dog challenged with S. equi subsp. zooepidemicus (C), from a dog challenged with CIV and S. equi subsp. zooepidemicus (D), and from a CIV-vaccinated dog challenged with CIV and S. equi subsp. zooepidemicus (E) are shown.
Tables
- Table 1.
Seroconversion in four groups of dogs
Treatment group CIV vaccinationa Challenge HI antibody titer (GMT)b Prevaccination Prechallenge 14 days postchallenge 1 No CIV <10 <10 226 ± 200 2 No S. equi subsp. zooepidemicus <10 <10 <10 3 No CIV + S. equi subsp. zooepidemicus <10 <10 278 ± 339 4 Yes CIV + S. equi subsp. zooepidemicus <10 73 ± 65 6,378 ± 3,032
