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VETERINARY IMMUNOLOGY

Antigen Specificity of the Humoral Immune Response to Mycoplasma haemofelis Infection

Iain R. Peters, Chris R. Helps, Timothy J. Gruffydd-Jones, Michael J. Day, Séverine Tasker
Iain R. Peters
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom
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  • For correspondence: i.r.peters@bristol.ac.uk
Chris R. Helps
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom
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Timothy J. Gruffydd-Jones
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom
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Michael J. Day
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom
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Séverine Tasker
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom
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DOI: 10.1128/CVI.00136-10
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  • FIG. 1.
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    FIG. 1.

    Western blot of RBC membrane antigen (R) and M. haemofelis antigen (Hf) probed with plasma samples (a 1/250 dilution) collected preinfection (days −7 and 0) and postinfection (15, 29, 43, 57, 71, 85, 99, 111, 125, 139, and 153 dpi) from cat HF4. The calculated molecular masses for the major bands (kDa) are shown by arrowheads. The open triangle corresponds to the 71- to 72-kDa band 4.2 protein identified in both RBC and M. haemofelis antigen preparations.

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    FIG. 2.

    A Coomassie blue-stained polyacrylamide gel (A) of RBC membrane antigen (R) and a Western blot (B) of a pooled plasma sample (lane P; days −7 and 0, all cats) and RBC membrane antigen (lanes R) probed with the pooled plasma sample (1/250 dilution). The molecular mass (kDa) of each protein standard (lane S) is given on the left side. The calculated molecular mass (kDa) of each identified band is given on the right side with the likely RBC membrane proteins corresponding to the bands (3, 24). Three bands (*) were detected in the plasma sample incubated with the secondary antibody alone and correspond to immunoglobulin light chains (29 kDa), the γ heavy chain of IgG (54 kDa), and a faint band corresponding to μ heavy chain of IgM (75 kDa).

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  • TABLE 1.

    M. haemofelis antigens recognized during the humoral immune response in six cats experimentally infected with the organism

    Calculated molecular mass (kDa) of antigen bandNo. of animals reacting to individual bands at the following dpi:
    −7015294357718599111125139153
    970001244434344
    930000022222222
    78a0045566565566
    68a0056666666666
    60a0046666656555
    540011343346666
    530000111111111
    48a0044566666666
    38a0045566666666
    350000043444555
    29, 250016666666666
        Avg no. of bands detected003.85.76.58.58.28.28.28.78.58.88.8
    • ↵ a These bands were recognized by all cats and tended to be recognized first and were thus considered the major antigenic determinants.

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Antigen Specificity of the Humoral Immune Response to Mycoplasma haemofelis Infection
Iain R. Peters, Chris R. Helps, Timothy J. Gruffydd-Jones, Michael J. Day, Séverine Tasker
Clinical and Vaccine Immunology Jul 2010, 17 (8) 1238-1243; DOI: 10.1128/CVI.00136-10

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Antigen Specificity of the Humoral Immune Response to Mycoplasma haemofelis Infection
Iain R. Peters, Chris R. Helps, Timothy J. Gruffydd-Jones, Michael J. Day, Séverine Tasker
Clinical and Vaccine Immunology Jul 2010, 17 (8) 1238-1243; DOI: 10.1128/CVI.00136-10
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    • ABSTRACT
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KEYWORDS

Antibodies, Bacterial
Antigens, Bacterial
Cat Diseases
Mycoplasma
Mycoplasma Infections

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