Article Figures & Data
Figures
- FIG. 1.
Western blot of RBC membrane antigen (R) and M. haemofelis antigen (Hf) probed with plasma samples (a 1/250 dilution) collected preinfection (days −7 and 0) and postinfection (15, 29, 43, 57, 71, 85, 99, 111, 125, 139, and 153 dpi) from cat HF4. The calculated molecular masses for the major bands (kDa) are shown by arrowheads. The open triangle corresponds to the 71- to 72-kDa band 4.2 protein identified in both RBC and M. haemofelis antigen preparations.
- FIG. 2.
A Coomassie blue-stained polyacrylamide gel (A) of RBC membrane antigen (R) and a Western blot (B) of a pooled plasma sample (lane P; days −7 and 0, all cats) and RBC membrane antigen (lanes R) probed with the pooled plasma sample (1/250 dilution). The molecular mass (kDa) of each protein standard (lane S) is given on the left side. The calculated molecular mass (kDa) of each identified band is given on the right side with the likely RBC membrane proteins corresponding to the bands (3, 24). Three bands (*) were detected in the plasma sample incubated with the secondary antibody alone and correspond to immunoglobulin light chains (29 kDa), the γ heavy chain of IgG (54 kDa), and a faint band corresponding to μ heavy chain of IgM (75 kDa).
Tables
- TABLE 1.
M. haemofelis antigens recognized during the humoral immune response in six cats experimentally infected with the organism
Calculated molecular mass (kDa) of antigen band No. of animals reacting to individual bands at the following dpi: −7 0 15 29 43 57 71 85 99 111 125 139 153 97 0 0 0 1 2 4 4 4 3 4 3 4 4 93 0 0 0 0 0 2 2 2 2 2 2 2 2 78a 0 0 4 5 5 6 6 5 6 5 5 6 6 68a 0 0 5 6 6 6 6 6 6 6 6 6 6 60a 0 0 4 6 6 6 6 6 5 6 5 5 5 54 0 0 1 1 3 4 3 3 4 6 6 6 6 53 0 0 0 0 1 1 1 1 1 1 1 1 1 48a 0 0 4 4 5 6 6 6 6 6 6 6 6 38a 0 0 4 5 5 6 6 6 6 6 6 6 6 35 0 0 0 0 0 4 3 4 4 4 5 5 5 29, 25 0 0 1 6 6 6 6 6 6 6 6 6 6 Avg no. of bands detected 0 0 3.8 5.7 6.5 8.5 8.2 8.2 8.2 8.7 8.5 8.8 8.8 ↵ a These bands were recognized by all cats and tended to be recognized first and were thus considered the major antigenic determinants.
