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VACCINE RESEARCH

Blocking Effect of a Monoclonal Antibody against Recombinant Pvs25 on Sporozoite Development in Anopheles sinensis

Sung-Ung Moon, Hyung-Hwan Kim, Tong-Soo Kim, Kyung-Mi Choi, Chang-Mi Oh, Yong-Joo Ahn, Seo-Kyoung Hwang, Youngjoo Sohn, E-Hyun Shin, Hyuck Kim, Hyeong-Woo Lee
Sung-Ung Moon
1Division of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea
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Hyung-Hwan Kim
2Vascular Medicine Research Unit, Brigham and Women's Hospital/Harvard Medical School, Cambridge, Massachusetts 02139
3International Research Center of Bioscience & Biotechnology, Jungwon University, Goesan 367-805, Republic of Korea
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Tong-Soo Kim
4Department of Parasitology, College of Medicine, Inha University, Incheon 405-751, Republic of Korea
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Kyung-Mi Choi
1Division of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea
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Chang-Mi Oh
1Division of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea
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Yong-Joo Ahn
2Vascular Medicine Research Unit, Brigham and Women's Hospital/Harvard Medical School, Cambridge, Massachusetts 02139
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Seo-Kyoung Hwang
2Vascular Medicine Research Unit, Brigham and Women's Hospital/Harvard Medical School, Cambridge, Massachusetts 02139
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Youngjoo Sohn
5Department of Gynecology, College of Oriental Medicine, Sangji University, Wonju 220-717, Republic of Korea
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E-Hyun Shin
6Division of Medical Entomology, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea
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Hyuck Kim
3International Research Center of Bioscience & Biotechnology, Jungwon University, Goesan 367-805, Republic of Korea
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Hyeong-Woo Lee
1Division of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea
7Department of Pathology, University of Florida, J-566, 1600 SW Archer Road, Gainesville, Florida 32610
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  • For correspondence: rainlee67@yahoo.co.kr
DOI: 10.1128/CVI.00101-10
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ABSTRACT

To develop a vaccine to block the transmission of vivax malaria, the gene encoding the ookinete surface protein Pvs25 was cloned from a Korean malaria patient. The Pvs25 gene was 660 bp long, encoding 219 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, named rPvs25, showed a molecular mass of approximately 25 kDa by SDS-PAGE analysis. An anti-rPvs25 monoclonal antibody produced in BALB/c mice was able to inhibit sporozoite development in the mosquito Anopheles sinensis, which is known as the malaria transmission vector in the Republic of Korea. In addition, rPvs25 produced a relatively high antibody titer in BALB/c mice that lasted for more than 6 months. Based on these results, we suggest that recombinant Pvs25 could be a useful antigen in the development of a vaccine to prevent local malaria transmission in the Republic of Korea.

  • Copyright © 2010 American Society for Microbiology
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Blocking Effect of a Monoclonal Antibody against Recombinant Pvs25 on Sporozoite Development in Anopheles sinensis
Sung-Ung Moon, Hyung-Hwan Kim, Tong-Soo Kim, Kyung-Mi Choi, Chang-Mi Oh, Yong-Joo Ahn, Seo-Kyoung Hwang, Youngjoo Sohn, E-Hyun Shin, Hyuck Kim, Hyeong-Woo Lee
Clinical and Vaccine Immunology Jul 2010, 17 (8) 1183-1187; DOI: 10.1128/CVI.00101-10

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Blocking Effect of a Monoclonal Antibody against Recombinant Pvs25 on Sporozoite Development in Anopheles sinensis
Sung-Ung Moon, Hyung-Hwan Kim, Tong-Soo Kim, Kyung-Mi Choi, Chang-Mi Oh, Yong-Joo Ahn, Seo-Kyoung Hwang, Youngjoo Sohn, E-Hyun Shin, Hyuck Kim, Hyeong-Woo Lee
Clinical and Vaccine Immunology Jul 2010, 17 (8) 1183-1187; DOI: 10.1128/CVI.00101-10
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KEYWORDS

Anopheles
Antibodies, Monoclonal
Antibodies, Protozoan
Disease Vectors
Sporozoites

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