Article Figures & Data
Figures
- FIG. 1.
Experimental study design. PM, postmortem.
- FIG. 2.
Immune response to vaccination. (A) Frequency of PPD-specific IFN-γ-secreting cells measured by ELISpot assay. (B) Frequency of Ag85A-specific IFN-γ-secreting cells measured by ELISpot assay. (C) Quantity of IFN-γ secreted by PBMC during 6 days of culture of PBMC with PPD measured by ELISA. (D) Frequency of CD3+ T cells that secreted IFN-γ following stimulation with PPD. Vaccination with BCG at week 0 is indicated by the dotted line, and vaccination with MVA85A indicated by the dashed line at week 12.
- FIG. 3.
Polyfunctional T-cell analysis after vaccination. (A to C) Responding CD4+ T-cell populations following stimulation with Ag85A peptide pools. (D to H) Responding CD4+ T-cell populations following stimulation with PPD. (I and J) Responding CD8+ T cells following stimulation with PPD.
- FIG. 4.
Kaplan-Meier plot of survival of vaccinated and unvaccinated NHPs after challenge with M. tuberculosis.
- FIG. 5.
Measures of tuberculosis-induced pulmonary and clinical disease burden. (A) Ratio of lung volume to lesion volume determined by MR stereology. (B) Number of lesions in the lung enumerated by serial sectioning and manual counting. (C) Total pathology score determined by using a qualitative scoring system. (D) Scores attributed to the pulmonary component as part of the total pathology score. (E) Score attributed to the chest radiogram on the day of euthanasia. (F) Percent weight loss from peak postchallenge weight on the day of euthanasia. (G) ESR on the day of euthanasia. (H) Bacterial load in the lung.
- FIG. 6.
Immune responses after aerosol challenge with M. tuberculosis. (A to C) Frequency of mycobacterium-specific IFN-γ-secreting cells after challenge measured by ELISpot assay. (A) ESAT6; (B) Ag85A; (C) PPD. (D) Profile of IFN-γ secretion by PPD-stimulated whole blood measured by ELISA.
Tables
- TABLE 1.
Aerosol challenge doses of M. tuberculosis delivered to rhesus macaques
Vaccine group (regimen) Animal Presented dose (CFU) Estimated retained dose (CFU) A (BCG + MVA85A) K20 1,293 60 K61 898 40 K65 1,271 60 K69 858 40 K79 1,299 60 K86 812 40 B (BCG) K43 1,263 60 K44 1,368 65 K50 932 45 K52 1,275 60 K59 884 40 K80 817 40 C (no vaccine) K32 1,286 60 K47 1,237 60 K54 821 40 K62 821 40 - TABLE 2.
Statistical analysis of the differences between test groups using different vaccine efficacy readoutsa
Readout P value Unvaccinated vs BCG Unvaccinated vs BCG-MVA85A BCG vs BCG-MVA85A Ratio of lesion vol to lung vol 0.011b 0.011b 0.631 No. of lesions 0.024c 0.199 0.337 Total pathology score 0.017b 0.198 0.33 Lung pathology score 0.079 0.083 0.466 Chest X ray 0.174 0.285 0.371 Bacterial load (CFU/g) 0.136 0.522 0.297 % wt loss 0.199 0.055 0.747 ESR 0.087 0.157 0.515
