Article Figures & Data
Figures
- FIG. 1.
Strategies implemented to improve antibody extraction from Ultracell sponges. (A) Detergents influence the SEAPNA in a concentration-dependent manner. Each condition was set up in triplicate, and the error bars were calculated from the standard deviations of the results of five separate experiments. (B) Recovery of mouse IgG1-TNP from Ultracell sponges incubated with BSA is dependent upon BSA concentration. BSA (0.5% to 0.00005%) was incubated with Ultracell sponges before they were spiked with mouse IgG1-TNP. The extracts were analyzed in a mouse IgG1 ELISA to determine recovery. (C) The addition of BSA to the EB slightly improved the recovery of mouse IgG1-TNP from Ultracell sponges. Mouse IgG1-TNP was spiked onto the sponges at 1,000 ng/ml. The extracts were analyzed in a mouse IgG1 ELISA to determine recovery. The results are representative of two separate experiments.
Tables
- TABLE 1.
Cumulative recoveries of V5 (anti-HPV16) spiked at 1/1,000 and 1/2,000 onto Merocel and Ultracell spongesa
Sponge type No. of sponges % V5 recovery Mean SD 95% CI Merocel Sterile 32 102 11.4 98.1-106 Participant 20 93.5 15.2 86.4-101 Ultracell Sterile 16 2.5 5.8 −0.64-5.6 Participant 35 61.2 49.3 44.3-78.2 ↵ a Mean, SD, and 95% CI values were calculated by combining the results obtained from sponges spiked with V5 at dilutions of 1/1,000 and 1/2,000.
