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MICROBIAL IMMUNOLOGY

Opsonophagocytic Activity and Other Serological Indications of Bordetella pertussis Infection in Military Recruits in Norway

Audun Aase, Tove Karin Herstad, Samuel Merino, Kari Torkildsen Brandsdal, Bjørn Peter Berdal, Erja M. Aleksandersen, Ingeborg S. Aaberge
Audun Aase
1Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway
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  • For correspondence: audun.aase@fhi.no
Tove Karin Herstad
1Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway
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Samuel Merino
1Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway
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Kari Torkildsen Brandsdal
2General Practice Branch, Armed Forces Medical Services, Oslo, Norway
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Bjørn Peter Berdal
3Institute of Microbiology, Armed Forces Medical Services, Oslo, Norway
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Erja M. Aleksandersen
4Ullevål University Hospital, Oslo, Norway
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Ingeborg S. Aaberge
1Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway
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DOI: 10.1128/CVI.00081-07
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  • FIG. 1.
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    FIG. 1.

    Flow cytometry histograms showing the respiratory-burst (RB) profiles of five different dilutions of positive S2 serum sample 659 (A to E) and one dilution of S1 serum sample 12 with low activity (F). The histograms are gated on the PMN population in a two-parameter dot plot of forward versus side scatter histograms to discriminate lymphocytes, monocytes, and PMNs (not shown). The horizontal bar defines the area of RB-positive cells, and the value above the bar is the percentage of positive PMNs. S2 serum sample 659 had an OPA titer of 256, whereas S1 serum sample 12 had a titer of <4. Overlaid in panel F is a profile of a negative control sample, i.e., dilution buffer instead of test serum (stippled histogram).

  • FIG. 2.
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    FIG. 2.

    Distribution of OPA titers and corresponding anti-PT IgG levels. The bars represent the number of samples at each OPA titer, and the diamonds reflect the geometric mean Serion PT IgG level at each OPA titer. All serum samples, both S1 and S2, are included.

Tables

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  • TABLE 1.

    Serion anti-PT IgG levels in paired serum samples collected from all participantsa

    Sample groupb (no. of samples)Geometric mean activity (FDA U/ml)d
    S1S2
    1 (361)6.0 (5.6, 6.5)4.8 (4.5, 5.2)
    2c (64)37.8 (31.7, 45.2)37.4 (33.5, 41.8)
    3 (39)165.4 (142.3, 192.3)100.0 (82.9, 120.5)
    • ↵ a Samples were collected at entry into military service (S1) and 8 months later (S2). Paired serum samples were grouped on the basis of Serion PT IgG activity.

    • ↵ b Serion PT IgG levels were as follows: for group 1, <30 FDA U/ml in both S1 and S2; for group 2, 30 to 80 FDA U/ml in S1 and/or S2; and for group 3, >80 FDA U/ml in S1.

    • ↵ c Also included in group 2 is the one subject with seroconversion during service (reaching 103.0 FDA U/ml in the S2 sample).

    • ↵ d Values in parentheses are 95% CIs.

  • TABLE 2.

    Geometric mean activities measured by the different assaysa

    Sample group (no. of samples)bGeometric mean activity (95% CI)
    Serion PT IgG (FDA U/ml)Pert FHA IgG (% of cutoff)Pert FHA IgA (% of cutoff)Pert PT IgG (% of cutoff)Pert PT IgA (% of cutoff)OPA against live bacteria (titer)C3b against live bacteria (AU/ml)IgG against live bacteria (AU/ml)
    1 (92)4.9 (4.1, 5.9)126.1 (113.1, 140.4)54.6 (49.3, 60.6)101.0 (92.1, 110.8)82.5 (76.6, 88.8)49.2 (38.1, 63.5)232.6 (206.0, 262.6)62.6 (50.1, 78.3)
    2 (89)47.7 (45.2, 50.5)246.6 (229.1, 265.5)92.0 (80.1, 105.7)267.4 (255.4, 279.9)80.5 (75.2, 86.2)227.8 (185.8, 279.2)520.3 (435.8, 621.1)375.4 (291.6, 483.2)
    3 (67)150.9 (135.3, 168.1)309.5 (283.5, 337.9)125.8 (108.0, 146.4)491.8 (466.2, 518.7)91.4 (83.8, 99.8)429.4 (343.6, 536.7)1,031.0 (799.2, 1,330.0)843.4 (620.4, 1,146.0)
    • ↵ a Pert, Pertusscan.

    • ↵ b All 248 serum samples (124 paired serum samples) were sorted by IgG anti-PT levels from the Serion assay. Groups were defined as described in footnote b of Table 1.

  • TABLE 3.

    Correlation between different assays of the 248 serum samples testeda

    TestPearson's correlation coefficient (r) with:
    Serion PT IgGPertusscan FHA IgGPertusscan FHA IgAPertusscan PT IgGPertusscan PT IgAIgG against live bacteriaOPA against live bacteria
    Pertusscan FHA IgG0.72
    Pertusscan FHA IgA0.510.65
    Pertusscan PT IgG0.950.720.53
    Pertusscan PT IgA0.14b0.19c0.490.25
    IgG live bacteria0.670.720.560.680.22d
    OPA live bacteria0.680.790.640.690.270.83
    C3b live bacteria0.590.520.470.610.21e0.760.70
    • ↵ a Unless specified otherwise, P < 0.0001.

    • ↵ b P = 0.03.

    • ↵ c P = 0.0027.

    • ↵ d P = 0.0006.

    • ↵ e P = 0.001.

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Opsonophagocytic Activity and Other Serological Indications of Bordetella pertussis Infection in Military Recruits in Norway
Audun Aase, Tove Karin Herstad, Samuel Merino, Kari Torkildsen Brandsdal, Bjørn Peter Berdal, Erja M. Aleksandersen, Ingeborg S. Aaberge
Clinical and Vaccine Immunology Jul 2007, 14 (7) 855-862; DOI: 10.1128/CVI.00081-07

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Opsonophagocytic Activity and Other Serological Indications of Bordetella pertussis Infection in Military Recruits in Norway
Audun Aase, Tove Karin Herstad, Samuel Merino, Kari Torkildsen Brandsdal, Bjørn Peter Berdal, Erja M. Aleksandersen, Ingeborg S. Aaberge
Clinical and Vaccine Immunology Jul 2007, 14 (7) 855-862; DOI: 10.1128/CVI.00081-07
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KEYWORDS

Bordetella pertussis
Military Personnel
Pertussis Vaccine
phagocytosis
Whooping Cough

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