Article Figures & Data
Figures
- FIG. 1.
Colonization of the lungs by B. pertussis. Mice were subcutaneously injected with (H) 1/5, (M) 1/25, or (L) 1/125 HD DTaP vaccine plus aluminum (alum) or MPL or (C) with the adjuvants only twice before intranasal B. pertussis infection. At 3 and 7 days after infection the lungs were excised and the number of viable B. pertussis organisms in the right lung lobes was determined. Each symbol represents the number of bacteria in the lung of an individual mouse; horizontal lines represent the group average. Nonboxed numbers show P values when the different vaccine doses were compared for the same adjuvant and day of killing. Boxed numbers show P values when the different adjuvants are compared for the same vaccine dose and day of killing. ANOVA was followed by t test. The results of a single representative experiment of three are shown.
- FIG. 2.
Ptx-specific IgG in serum. Mice were injected s.c. with 1/5 HD DTaP vaccine plus aluminum (alum) or MPL or with the adjuvants only twice before intranasal B. pertussis infection. At 3 and 7 days after infection, the mice were euthanized. Serum was taken, and serial dilutions of test and positive control sera were tested for Ptx-specific IgG. The data are indicated as the means ± standard errors of the means (n = 5). ANOVA was followed by t test. The results of a single representative experiment of two are shown.
- FIG. 3.
Lung eosinophilia. Mice were injected s.c. with 1/5 HD DTaP vaccine plus aluminum (alum) or MPL or with the adjuvants only twice before intranasal B. pertussis infection. At 3 days after infection, the left lung lobes were excised. Each symbol represents an individual mouse; horizontal lines represent the group average. The Mann-Whitney U test was used. The results of a single representative experiment of two are shown.
- FIG. 4.
Total serum IgE. Mice were injected s.c. with 1/5 HD DTaP vaccine plus aluminum (alum) or MPL or with the adjuvants only twice before intranasal B. pertussis infection. At 3 days after infection, serum was taken and assayed for total IgE. The data are indicated as the means ± standard errors of the means (n = 6). ANOVA was followed by use of the Bonferroni correction. The results of a single representative experiment of two are shown.
- FIG. 5.
(A) IL-4 production by ex vivo ConA-stimulated bronchial LN cells. Mice were injected s.c. with 1/5 HD DTaP vaccine plus aluminum (alum) or MPL or with the adjuvants only twice before intranasal B. pertussis infection. Three days after infection, the bronchial LNs were excised, the cells were cultured with ConA for 24 h, and the supernatants were analyzed for their cytokine contents. (B) IL-5 production by splenocytes stimulated ex vivo with heat-killed B. pertussis. The splenocytes were cultured with heat-killed B. pertussis for 72 h, and the supernatants were analyzed for their cytokine contents. The data are indicated as the means ± standard errors of the means (n = 6). ANOVA was followed by use of the Bonferroni correction. The results of a single representative experiment of two are shown.
- FIG. 6.
Colonization of the lungs by B. pertussis. Mice were injected s.c. with PBS or 1/10 HD DTaP vaccine plus aluminum, LpxL2 LPS, or MPL twice before intranasal B. pertussis infection. At 5 days after infection the lungs were excised, and the number of viable B. pertussis organisms was determined. Each symbol represents the number of bacteria in the lung of an individual mouse; horizontal lines represent the group average. ANOVA was followed by t test. The results of a single representative experiment of two are shown.
- FIG. 7.
BALF eosinophil numbers. Mice were injected s.c. with 1/10 HD DTaP vaccine plus aluminum, LpxL2 LPS, or MPL or with PBS twice before intranasal B. pertussis infection. At 5 days after infection, lung lavage was performed and the BALF cells were counted and visually differentiated. The data are indicated as the means ± standard errors of the means (n = 6). ANOVA was followed by use of the Bonferroni correction. The results of a single representative experiment of two are shown.
- FIG. 8.
Serum IL-6. Mice were injected s.c. with 1/10 HD wP vaccine; 1/10 HD DTaP vaccine plus aluminum, LpxL2 LPS, or MPL; or PBS. Serum IL-6 concentrations were determined 4 h postimmunization. The data are indicated as the means ± standard errors of the means (n = 7). ANOVA was followed by use of the Bonferroni correction. The results of a single representative experiment of two are shown.
