Article Figures & Data
Figures
- FIG. 1.
Comparison of standard 27-Ga needle to 34-Ga microneedle. The displayed microneedle has an inner diameter of 76 μm, an outer diameter of 178 μm, and a total exposed length of 1.0 mm.
- FIG. 2.
Serum antibody response in rats (n = 4 per group) following immunization with 1, 0.1, or 0.01 μg of whole inactivated influenza virus A/PR/8/34 on days 0, 21, and 42. Influenza virus-specific antibodies were measured by ELISA on (A) day 21, (B) day 42, and (C) day 56. Displayed are ELISA GMTs ± standard deviation.
- FIG. 3.
Rats (n = 10 per group) were immunized with either a high dose (100 μl of undiluted vaccine) or a low dose (100 μl of vaccine diluted 1:10) of trivalent split-virion influenza vaccine (Fluzone 2003-2004 formulation) and analyzed for serum antibody response 21 days later. Bars represent group GMT, and open symbols represent the responses from individual animals. (A) ELISA response against the H1N1 (A/NC/20/99) strain. (B) HAI response against the H1N1 (A/NC/20/99) strain. (C) ELISA response against the H3N2 (A/Pan/2007/99) strain. (D) HAI response against the H3N2 (A/Pan/2007/99) strain. (E) ELISA response against the B (B/HK/1434/02) strain. Due to insufficient quantities of sera from some animals, for analysis of ELISA titers against the B strain, only seven individuals from the i.d. “low-dose” group and nine animals from the i.m. “low-dose” group were analyzed. (F) HAI response against the B (B/HK/1434/02) strain.
- FIG. 4.
Luciferase activity in skin or muscle following i.d. or i.m. administration of either 50 μg or 5 μg of pCMV-Luc (n = 4 rats per group). As a negative control, rats were injected with the unrelated reporter plasmid, pCMV-β. Tissues were collected 24 h postdelivery and analyzed by luciferase assay for reporter gene expression. Luciferase activity is expressed as mean increase in RLU over background luciferase activity from tissue sites that had been injected with pCMV-β. Bars represent group means, and open symbols represent responses from individual animals.
- FIG. 5.
Serum antibody response in rats (n = 5 per group) following immunization with 50, 10, 5, or 1 μg of pCMV-HA on days 0, 21, and 42. Antibodies against whole inactivated influenza virus A/PR/8/34 were measured by ELISA on (A) day 21, (B) day 42, and (C) day 56. Displayed are ELISA GMTs ± standard deviation.
