Article Figures & Data
Figures
- FIG. 1.
ELISPOT assay determining the effects of increasing the number of E6-vac-infected HLA-B57-positive monocytes and the MOI for E6-vac. The adjacent white bars represent the corresponding WR-vac-negative controls. The numbers of SFU increased as more monocytes were added. The differences between the results seen with 1 × 104 monocytes and with 3 × 105 monocytes were statistically significant at an MOI of 10 (P = 0.0002) and an MOI of 20 (P = 0.01). Furthermore, at smaller numbers of monocytes (1 × 104 and 5 × 104), the increases in MOI from an MOI of 5 to an MOI of 20 were statistically significant at 1 × 104 (P = 0.02) and at 5 × 104 (P = 0.01). The error bars represent standard errors of the means. Results representative of three experiments are shown.
- FIG. 2.
ELISPOT assay evaluating the effects of adding exogenous rhIL-2 and rhIL-7. The adjacent white bars are the corresponding WR-vac-negative controls. Statistically significant increases in the numbers of SFU were demonstrated when rhIL-2 was added alone compared to the results seen in the absence of rhIL-2 and rhIL-7 (P = 0.03 at 10 units/ml, P = 0.02 at 20 units/ml, and P = 0.03 at 30 units/ml). However, the addition of rhIL-7 alone did not yield statistically significant results. Interestingly, the addition of both rhIL-2 and rhIL-7 may have an additive effect although the effect may be only very slightly above that seen with rhIL-2 alone. The error bars represent standard errors of the means. Results representative of two experiments are shown.
- FIG. 3.
Serial dilution of HPV-specific CD8+ T-cell clone cells. The E6 52-61-specific CD8+ T-cell clone cells were serially diluted 8 times by a factor of 2 starting from 1,000 cells. Infected monocytes were plated at either 1 × 105 per well or 2 × 105 per well. The correlations between the numbers of T-cell clone cells added and the numbers of SFU detected were nearly linear. The error bars represent standard errors of the means.
Tables
- TABLE 1.
Subject age, sex, pap smear, biopsy, and ELISPOT resultsa
Subject Category Age (yr) Sex Initial Pap result(s) Biopsy result Treatment Pap result at blood draw ELISPOT resultb E6-vac E7-vac 1 Patient 45 F ASCUS, HPV+ ND None − 630 607 2 Patient 31 F HSIL CIN1 LEEP LSIL 180 − 3 Patient 21 F LSIL ND Cryotherapy − − − 4 Patient 37 F ASCUS, HPV+ CIN1 LEEP LSIL − − 5 Patient 29 F ASCUS × 2 CIN2/3 LEEP HSIL − − 6 Patient 27 F HSIL CIN1 LEEP ASCUS − − 7 Healthy 40 F NA NA NA NA − − 8 Healthy 38 F NA NA NA NA − − 9 Healthy 34 M NA NA NA NA − − 10 Healthy 40 F NA NA NA NA − − 11 Healthy 24 M NA NA NA NA − − 12 Healthy 23 M NA NA NA NA − − 13 Healthy 49 F NA NA NA NA − − 14 Healthy 41 F NA NA NA NA − − 15 Healthy 41 M NA NA NA NA − − 16 Healthy 45 M NA NA NA NA − − 17 Healthy 45 F NA NA NA NA 197 107 18 Healthy 52 F NA NA NA NA − − ↵ a F, female; M, male; ASCUS, atypical cells of undetermined significance; ND, not done; HSIL, high-grade squamous intraepithelial lesion; CIN1 and CIN2, cervical intraepithelial neoplasia grades 1 and 2; NA, not applicable; LEEP, loop electrosurgical excision procedure; LSIL, low-grade squamous epithelial lesion. −, negative result.
↵ b Numbers of spot-forming units per million PBMC after subtracting the number calculated for the background to WR-vac in samples with positive results.
