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CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY

Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory

Janet C. Patton, Eveline Akkers, Ashraf H. Coovadia, Tammy M. Meyers, Wendy S. Stevens, Gayle G. Sherman
Janet C. Patton
Wits Pediatric HIV Clinics, Wits Health Consortium
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Eveline Akkers
Departments of Molecular Medicine and HematologyNational Health Laboratory Service, Johannesburg, Gauteng, South Africa
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Ashraf H. Coovadia
Wits Pediatric HIV Clinics, Wits Health ConsortiumPediatrics, University of the Witwatersrand, Johannesburg, Gauteng, South Africa
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Tammy M. Meyers
Wits Pediatric HIV Clinics, Wits Health ConsortiumPediatrics, University of the Witwatersrand, Johannesburg, Gauteng, South Africa
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Wendy S. Stevens
Departments of Molecular Medicine and HematologyNational Health Laboratory Service, Johannesburg, Gauteng, South Africa
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Gayle G. Sherman
Wits Pediatric HIV Clinics, Wits Health ConsortiumDepartments of Molecular Medicine and HematologyNational Health Laboratory Service, Johannesburg, Gauteng, South Africa
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  • For correspondence: gayle.sherman@nhls.ac.za
DOI: 10.1128/CVI.00223-06
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ABSTRACT

The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.

FOOTNOTES

    • Received 15 June 2006.
    • Returned for modification 20 September 2006.
    • Accepted 1 December 2006.
  • ↵▿ Published ahead of print on 13 December 2006.

  • American Society for Microbiology
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Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory
Janet C. Patton, Eveline Akkers, Ashraf H. Coovadia, Tammy M. Meyers, Wendy S. Stevens, Gayle G. Sherman
Clinical and Vaccine Immunology Feb 2007, 14 (2) 201-203; DOI: 10.1128/CVI.00223-06

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Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory
Janet C. Patton, Eveline Akkers, Ashraf H. Coovadia, Tammy M. Meyers, Wendy S. Stevens, Gayle G. Sherman
Clinical and Vaccine Immunology Feb 2007, 14 (2) 201-203; DOI: 10.1128/CVI.00223-06
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