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MICROBIAL IMMUNOLOGY

Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

Blanca Velázquez, Hugo Massaldi, Julio Battistoni, José A. Chabalgoity
Blanca Velázquez
1Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay
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Hugo Massaldi
1Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay
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Julio Battistoni
2Laboratory of Immunotechnology, Department of Immunology, School of Chemistry-School of Science, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay
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José A. Chabalgoity
1Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay
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  • For correspondence: jachabal@higiene.edu.uy
DOI: 10.1128/CDLI.12.5.683-684.2005
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    Analysis of the purified extract by immunoblotting. The material eluted from the glutathione-Sepharose column was separated according to molecular weight on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immediately transferred to nitrocellulose for analysis by antigenic recognition. Nitrocellulose strips were assessed with the following: lane 1, anti-SLO human serum 1/500 and developed with an anti-human IgG conjugated to alkaline phosphatase 1/1,000; lane 2, mouse anti-GST serum 1/200 and developed with an anti-mouse IgG conjugated to alkaline phosphatase 1/1,000; lane 3, mouse anti-GST serum previously incubated with GST (0.0075 mg/ml) and developed with anti-mouse IgG conjugated to alkaline phosphatase 1/1,000; lane 4, negative control.

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    FIG. 2.

    Correlation between anti-SLO antibody titers measured by ELISA and by latex agglutination. ELISA and latex results were plotted, and their statistical relationships were assessed using log-transformed values.

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Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays
Blanca Velázquez, Hugo Massaldi, Julio Battistoni, José A. Chabalgoity
Clinical and Diagnostic Laboratory Immunology May 2005, 12 (5) 683-684; DOI: 10.1128/CDLI.12.5.683-684.2005

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Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays
Blanca Velázquez, Hugo Massaldi, Julio Battistoni, José A. Chabalgoity
Clinical and Diagnostic Laboratory Immunology May 2005, 12 (5) 683-684; DOI: 10.1128/CDLI.12.5.683-684.2005
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