Article Figures & Data
Figures
- FIG. 1.
Analysis of the purified extract by immunoblotting. The material eluted from the glutathione-Sepharose column was separated according to molecular weight on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immediately transferred to nitrocellulose for analysis by antigenic recognition. Nitrocellulose strips were assessed with the following: lane 1, anti-SLO human serum 1/500 and developed with an anti-human IgG conjugated to alkaline phosphatase 1/1,000; lane 2, mouse anti-GST serum 1/200 and developed with an anti-mouse IgG conjugated to alkaline phosphatase 1/1,000; lane 3, mouse anti-GST serum previously incubated with GST (0.0075 mg/ml) and developed with anti-mouse IgG conjugated to alkaline phosphatase 1/1,000; lane 4, negative control.
- FIG. 2.
Correlation between anti-SLO antibody titers measured by ELISA and by latex agglutination. ELISA and latex results were plotted, and their statistical relationships were assessed using log-transformed values.
