Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Clinical and Vaccine Immunology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
MICROBIAL IMMUNOLOGY

Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus

Maofeng Qiu, Jin Wang, Hongxia Wang, Zeliang Chen, Erhei Dai, Zhaobiao Guo, Xiaoyi Wang, Xin Pang, Baoxing Fan, Jie Wen, Jian Wang, Ruifu Yang
Maofeng Qiu
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jin Wang
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hongxia Wang
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zeliang Chen
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erhei Dai
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhaobiao Guo
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaoyi Wang
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xin Pang
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Baoxing Fan
2PLA General Hospital
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jie Wen
3Beijing Genomics Institute, Chinese Academy of Sciences, Beijing, People's Republic of China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jian Wang
3Beijing Genomics Institute, Chinese Academy of Sciences, Beijing, People's Republic of China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruifu Yang
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: yangrf@nic.bmi.ac.cn
DOI: 10.1128/CDLI.12.3.474-476.2005
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Detection of antibodies to SARS-CoV by the N13-based antigen-capturing ELISA, the N-based ELISA, and the indirect ELISA based on whole-virus lysate in one twofold serially diluted serum sample from a SARS patient. The cutoff OD450 values for these assays were 0.17, 0.17, and 0.18, respectively.

Tables

  • Figures
  • TABLE 1.

    Detection of antibodies to the SARS-CoV in sera from patients with probable SARS by the N13-based antigen-capturing ELISA and the indirect ELISA based on a whole-virus lysate

    Day of sample collection after fever onsetNo. of samplesNo. (%) of samples positive by:
    Indirect ELISAAntigen- capturing ELISA
    IgGIgMIgG + IgM
    12-1471 (14.3)1 (14.3)1 (14.3)1 (14.3)
    15-212514 (56.0)9 (36.0)15 (60.0)13 (52.0)
    22-286055 (91.7)47 (78.3)56 (93.3)55 (91.7)
    29-359685 (88.5)62 (64.6)88 (91.7)91 (94.8)
    36-425145 (88.2)30 (58.8)46 (90.2)51 (100.0)
    43-492623 (88.5)15 (57.7)23 (88.5)24 (92.3)
    50-721512 (80.0)6 (40.0)12 (80.0)15 (100.0)
    Total280235 (83.9)170 (60.7)241 (86.1)250 (89.3)
PreviousNext
Back to top
Download PDF
Citation Tools
Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus
Maofeng Qiu, Jin Wang, Hongxia Wang, Zeliang Chen, Erhei Dai, Zhaobiao Guo, Xiaoyi Wang, Xin Pang, Baoxing Fan, Jie Wen, Jian Wang, Ruifu Yang
Clinical and Diagnostic Laboratory Immunology Mar 2005, 12 (3) 474-476; DOI: 10.1128/CDLI.12.3.474-476.2005

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Clinical and Vaccine Immunology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus
(Your Name) has forwarded a page to you from Clinical and Vaccine Immunology
(Your Name) thought you would be interested in this article in Clinical and Vaccine Immunology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus
Maofeng Qiu, Jin Wang, Hongxia Wang, Zeliang Chen, Erhei Dai, Zhaobiao Guo, Xiaoyi Wang, Xin Pang, Baoxing Fan, Jie Wen, Jian Wang, Ruifu Yang
Clinical and Diagnostic Laboratory Immunology Mar 2005, 12 (3) 474-476; DOI: 10.1128/CDLI.12.3.474-476.2005
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About CVI
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1556-6811; Online ISSN: 1556-679X