DOI: 10.1128/CDLI.12.3.474-476.2005
Article Figures & Data
Figures
- FIG. 1.
Detection of antibodies to SARS-CoV by the N13-based antigen-capturing ELISA, the N-based ELISA, and the indirect ELISA based on whole-virus lysate in one twofold serially diluted serum sample from a SARS patient. The cutoff OD450 values for these assays were 0.17, 0.17, and 0.18, respectively.
Tables
- TABLE 1.
Detection of antibodies to the SARS-CoV in sera from patients with probable SARS by the N13-based antigen-capturing ELISA and the indirect ELISA based on a whole-virus lysate
Day of sample collection after fever onset No. of samples No. (%) of samples positive by: Indirect ELISA Antigen- capturing ELISA IgG IgM IgG + IgM 12-14 7 1 (14.3) 1 (14.3) 1 (14.3) 1 (14.3) 15-21 25 14 (56.0) 9 (36.0) 15 (60.0) 13 (52.0) 22-28 60 55 (91.7) 47 (78.3) 56 (93.3) 55 (91.7) 29-35 96 85 (88.5) 62 (64.6) 88 (91.7) 91 (94.8) 36-42 51 45 (88.2) 30 (58.8) 46 (90.2) 51 (100.0) 43-49 26 23 (88.5) 15 (57.7) 23 (88.5) 24 (92.3) 50-72 15 12 (80.0) 6 (40.0) 12 (80.0) 15 (100.0) Total 280 235 (83.9) 170 (60.7) 241 (86.1) 250 (89.3)
Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus
