DOI: 10.1128/CDLI.12.10.1231-1234.2005
Article Figures & Data
Tables
- TABLE 1.
RPR and treponemal test reactivities detected in the panel of negative control seraa
Specimen category (no. of samples) % Reactivity (no. of samples reactive or equivocal/total no. tested) RPR CLIA EIA TPHA WB Blood donors (2494) 2.77 (69/2,494) 0.12 (3/2,494) 0.04 (1/2,494) 0 0.04 (1/2,494) Lyme disease (30) 0 0 0 3.33 (1/30) 0 Streptococcus pyogenes infection (10) 10 (1/10) 0 0 0 0 Mononucleosis (10) 10 (1/10) 0 0 0 0 Pregnant women (5) 20 (1/5) 0 0 0 0 ANA positive (16) 12.5 (2/16) 0 0 0 0 RF positive (10) 10 (1/10) 0 0 0 0 T. denticola positive (15) 0 0 0 0 0 Total (2,590) 2.90 (75/2,590) 0.12 (3/2,590) 0.04 (1/2,590) 0.04 (1/2,590) 0.04 (1/2,590) ↵a Treponemal tests used were LIAISON Treponema Screen, syphilis screening recombinant EIA, TPHA, and WB. Negative control sera were samples from blood donors and cross-reacting samples. ANA, antinuclear antibody; RF, rheumatoid factor.
- TABLE 2.
Test reactivities in relation to stage of syphilis and treatment status
Stage of disease and treatment status (no. of samples) % Reactivity (no. of samples reactive/total no. tested)a CLIA EIA TPHA WB RPR Untreated primary (7) 100 57.1 (4/7) 57.1 (4/7) 100 85.7 (6/7) Untreated secondary (31) 100 100 100 100 96.8 (30/31) Treated latent (77) 98.7 (76/77) 96.1 (74/77) 96.1 (74/77) 100 6.5 (5/77) Untreated cardiovascular (5) 100 100 100 100 100 Untreated neurosyphilis (6) 100 100 100 100 100 Untreated congenital (5) 100 100 100 100 100 Total (131) 99.2 (130/131) 95.4 (125/131) 94.7 (124/131) 100 43.5 (57/131) ↵a Tests compared were LIAISON Treponema Screen, syphilis screening recombinant EIA, TPHA, WB, and RPR.
- TABLE 3.
Sensitivities and specificities of tests, as detected in the retrospective study
Test % Sensitivity % Specificity LIAISON Treponema Screen 99.2 99.9 Syphilis screening recombinant EIA 95.4 99.9 TPHA 94.7 99.9 WB 100 99.9 RPRa 96.3 97.1 ↵a RPR sensitivity was calculated considering only the results obtained by testing the 54 sera of untreated syphilis patients.