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MICROBIAL IMMUNOLOGY

Evaluation of LIAISON Treponema Screen, a Novel Recombinant Antigen-Based Chemiluminescence Immunoassay for Laboratory Diagnosis of Syphilis

Antonella Marangoni, Vittorio Sambri, Silvia Accardo, Francesca Cavrini, Antonietta D'Antuono, Alessandra Moroni, Elisa Storni, Roberto Cevenini
Antonella Marangoni
Microbiology Section
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Vittorio Sambri
Microbiology Section
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  • For correspondence: vsambri@med.unibo.it
Silvia Accardo
Microbiology Section
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Francesca Cavrini
Centro di Riferimento Regionale per le Emergenze Microbiologiche, Ospedale Policlinico S. Orsola, Bologna, Italy
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Antonietta D'Antuono
Dermatology Section, DMCSS, University of Bologna
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Alessandra Moroni
Microbiology Section
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Elisa Storni
Centro di Riferimento Regionale per le Emergenze Microbiologiche, Ospedale Policlinico S. Orsola, Bologna, Italy
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Roberto Cevenini
Microbiology Section
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DOI: 10.1128/CDLI.12.10.1231-1234.2005
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Tables

  • TABLE 1.

    RPR and treponemal test reactivities detected in the panel of negative control seraa

    Specimen category (no. of samples)% Reactivity (no. of samples reactive or equivocal/total no. tested)
    RPRCLIAEIATPHAWB
    Blood donors (2494)2.77 (69/2,494)0.12 (3/2,494)0.04 (1/2,494)00.04 (1/2,494)
    Lyme disease (30)0003.33 (1/30)0
    Streptococcus pyogenes infection (10)10 (1/10)0000
    Mononucleosis (10)10 (1/10)0000
    Pregnant women (5)20 (1/5)0000
    ANA positive (16)12.5 (2/16)0000
    RF positive (10)10 (1/10)0000
    T. denticola positive (15)00000
    Total (2,590)2.90 (75/2,590)0.12 (3/2,590)0.04 (1/2,590)0.04 (1/2,590)0.04 (1/2,590)
    • ↵a Treponemal tests used were LIAISON Treponema Screen, syphilis screening recombinant EIA, TPHA, and WB. Negative control sera were samples from blood donors and cross-reacting samples. ANA, antinuclear antibody; RF, rheumatoid factor.

  • TABLE 2.

    Test reactivities in relation to stage of syphilis and treatment status

    Stage of disease and treatment status (no. of samples)% Reactivity (no. of samples reactive/total no. tested)a
    CLIAEIATPHAWBRPR
    Untreated primary (7)10057.1 (4/7)57.1 (4/7)10085.7 (6/7)
    Untreated secondary (31)10010010010096.8 (30/31)
    Treated latent (77)98.7 (76/77)96.1 (74/77)96.1 (74/77)1006.5 (5/77)
    Untreated cardiovascular (5)100100100100100
    Untreated neurosyphilis (6)100100100100100
    Untreated congenital (5)100100100100100
    Total (131)99.2 (130/131)95.4 (125/131)94.7 (124/131)10043.5 (57/131)
    • ↵a Tests compared were LIAISON Treponema Screen, syphilis screening recombinant EIA, TPHA, WB, and RPR.

  • TABLE 3.

    Sensitivities and specificities of tests, as detected in the retrospective study

    Test% Sensitivity% Specificity
    LIAISON Treponema Screen99.299.9
    Syphilis screening recombinant EIA95.499.9
    TPHA94.799.9
    WB10099.9
    RPRa96.397.1
    • ↵a RPR sensitivity was calculated considering only the results obtained by testing the 54 sera of untreated syphilis patients.

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Evaluation of LIAISON Treponema Screen, a Novel Recombinant Antigen-Based Chemiluminescence Immunoassay for Laboratory Diagnosis of Syphilis
Antonella Marangoni, Vittorio Sambri, Silvia Accardo, Francesca Cavrini, Antonietta D'Antuono, Alessandra Moroni, Elisa Storni, Roberto Cevenini
Clinical and Diagnostic Laboratory Immunology Oct 2005, 12 (10) 1231-1234; DOI: 10.1128/CDLI.12.10.1231-1234.2005

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Evaluation of LIAISON Treponema Screen, a Novel Recombinant Antigen-Based Chemiluminescence Immunoassay for Laboratory Diagnosis of Syphilis
Antonella Marangoni, Vittorio Sambri, Silvia Accardo, Francesca Cavrini, Antonietta D'Antuono, Alessandra Moroni, Elisa Storni, Roberto Cevenini
Clinical and Diagnostic Laboratory Immunology Oct 2005, 12 (10) 1231-1234; DOI: 10.1128/CDLI.12.10.1231-1234.2005
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