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MICROBIAL IMMUNOLOGY

Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease

J. M. Rolain, F. Gouriet, M. Enea, M. Aboud, D. Raoult
J. M. Rolain
Unité des Rickettsies, CNRS UMR 6020A, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France
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F. Gouriet
Unité des Rickettsies, CNRS UMR 6020A, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France
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M. Enea
Unité des Rickettsies, CNRS UMR 6020A, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France
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M. Aboud
Unité des Rickettsies, CNRS UMR 6020A, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France
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D. Raoult
Unité des Rickettsies, CNRS UMR 6020A, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France
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  • For correspondence: Didier.Raoult@medecine.univ-mrs.fr
DOI: 10.1128/CDLI.10.4.686-691.2003
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  • FIG. 1.
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    FIG. 1.

    Detection of B. henselae by IFD test in the lymph node of a patient with CSD by using a MAb directed against B. henselae as viewed by fluorescence microscopy (magnification, ×400).

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    FIG. 2.

    Sensitivities and specificities obtained with IFA and/or IFD tests. For sensitivity, the cutoff titer for IFA was set at ≥64, whereas for specificity, it was set at ≥128.

Tables

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  • TABLE 1.

    Oligonucleotide primers used for PCR amplification and sequencing

    Primer (reference gene)Nucleotide sequenceDetected organismReference(s)
    URBarto1 (its)CTT CGT TTC TCT TTC TTC A Bartonella species42
    URBarto2 (its)CTT CTC TTC ACA ATT TCA AT Bartonella species42
    PAPn1 (pap31)TTC TAG GAG TTG AAA CCG AT Bartonella species47, 48
    PAPn2 (pap31)GAA ACA CCA CCA GCA ACA TA Bartonella species47, 48
    PAPnS2 (pap31)GCA CCA GAC CAT TTT TCC TT
  • TABLE 2.

    Diagnoses for 189 patients with lymphadenopathy

    DiagnosisNo. (%) of patients
    CSD52 (28)
    Bacterial infection
        Coagulase-negative Staphylococcus13
        Staphylococcus aureus8
        Propionibacterium acnes3
        Streptococcus agalactiae2
        Pseudomonas aeruginosa1
        Enterococcus faecium1
        Methylobacterium sp.1
        Micrococcus sp.1
        Pasteurella sp.1
    Tuberculosis31 (16)
    Lymphoma18 (10)
    Cancer12 (6)
    Sarcoidosis5 (3)
    Q fever2 (1)
    Tularemia1 (1)
    B. quintana bacteremia1 (1)
    Bacillary angiomatosis1 (1)
    Candidiasis1 (1)
    Unknown62 (33)
    Total189 (100)
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Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease
J. M. Rolain, F. Gouriet, M. Enea, M. Aboud, D. Raoult
Clinical and Diagnostic Laboratory Immunology Jul 2003, 10 (4) 686-691; DOI: 10.1128/CDLI.10.4.686-691.2003

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Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease
J. M. Rolain, F. Gouriet, M. Enea, M. Aboud, D. Raoult
Clinical and Diagnostic Laboratory Immunology Jul 2003, 10 (4) 686-691; DOI: 10.1128/CDLI.10.4.686-691.2003
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