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MICROBIAL IMMUNOLOGY

Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks

Licel de los Angeles Rodríguez Lay, Osmany Larralde Díaz, Raiza Martínez Casanueva, Aidonis Gutiérrez Moreno
Licel de los Angeles Rodríguez Lay
Institute for Tropical Medicine “Pedro Kourí,” Department of Virology, National Reference Laboratory of Viral Hepatitis, Havana City, Cuba
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  • For correspondence: licel@ipk.sld.cu
Osmany Larralde Díaz
Institute for Tropical Medicine “Pedro Kourí,” Department of Virology, National Reference Laboratory of Viral Hepatitis, Havana City, Cuba
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Raiza Martínez Casanueva
Institute for Tropical Medicine “Pedro Kourí,” Department of Virology, National Reference Laboratory of Viral Hepatitis, Havana City, Cuba
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Aidonis Gutiérrez Moreno
Institute for Tropical Medicine “Pedro Kourí,” Department of Virology, National Reference Laboratory of Viral Hepatitis, Havana City, Cuba
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DOI: 10.1128/CDLI.10.3.492-494.2003
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Tables

  • TABLE 1.

    Discordant OD and T:N values for serum and urine samples from 20 patients

    SampleOD value for:T:N value for urine sampleaDiscordant results (serum/urine)
    Serum sampleUrine sample
    11.8690.0851.16a+/−
    20.7710.1011.13a+/−
    30.6650.0510.57+/−
    40.3390.0680.77+/−
    51.6030.0900.71+/−
    60.4880.0940.74+/−
    70.5540.0980.77+/−
    80.6960.0890.70+/−
    91.0890.1220.97a+/−
    100.5250.1051.1a+/−
    110.290.141.05a+/−
    121.610.110.84+/−
    130.3600.0931.13a+/−
    140.1130.6989.56−/+
    150.0910.1111.52−/+
    160.0791.55621.32−/+
    170.1410.4155.68−/+
    180.0820.0991.37−/+
    190.0780.1822.49−/+
    200.150.312.46−/+
    • ↵a T:N result localized in the “gray gone” (i.e., inconclusive).

  • TABLE 2.

    T:N values for urine samples stored at 4 and −70°C

    Urine sampleT:N value for urine sample at:
    Time zero1 mo2 mo3 mo6 mo
    4°C−70°C4°C−70°C4°C−70°C4°C−70°C
    14.83.33.915.294.514.83.153.23.7
    22.53.511.624.62.493.91.751a2.9
    35.64.844.535.316.055.85.4756.8
    45.033.382.833.163.782.92.53.53.2
    55.84.213.855.114.536.44.61.74.0
    66.73.464.25.216.184.74.975.0
    76.84.84.294.415.676.043.356.5
    83.12.472.123.682.742.81.54.27.5
    95.94.684.696.016.365.75.97.59.2
    106.24.64.575.896.116.86.030.7a2.1
    114.83.243.294.424.474.53.50.9a1.4
    123.62.842.393.64.233.32.16.57.0
    133.091.921.762.52.662.31.80.6a1.9
    142.52.52.593.152.432.233.122.53.4
    156.036.038.51.215.370.758.90.6a3.3
    167.477.477.194.135.24.077.724.28.2
    Median5.003.953.904.234.554.194.143.384.76
    SD1.461.421.731.311.211.652.091.852.35
    • ↵a Sample tested negative after being stored for 6 months at 4°C.

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Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks
Licel de los Angeles Rodríguez Lay, Osmany Larralde Díaz, Raiza Martínez Casanueva, Aidonis Gutiérrez Moreno
Clinical and Diagnostic Laboratory Immunology May 2003, 10 (3) 492-494; DOI: 10.1128/CDLI.10.3.492-494.2003

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Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks
Licel de los Angeles Rodríguez Lay, Osmany Larralde Díaz, Raiza Martínez Casanueva, Aidonis Gutiérrez Moreno
Clinical and Diagnostic Laboratory Immunology May 2003, 10 (3) 492-494; DOI: 10.1128/CDLI.10.3.492-494.2003
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