Article Figures & Data
Figures
- FIG. 1.
Constitutive BPI mRNA expression in human dermal fibroblasts. Shown is the RT-PCR product from unstimulated human dermal fibroblasts as described in the text. In the upper image, the PCR product of BPI-specific RT-PCR (size, 841 bp) is shown. The lower image shows the product of GAPDH RT-PCR (housekeeping gene; size, 233 bp). Lane 1, human dermal fibroblasts; lane 2, negative-control sample (containing no RNA); lane 3, 100-bp marker.
- FIG. 2.
BPI total protein production by human dermal fibroblasts. (A) Shown is the Western blot band from culture supernatants from unstimulated human dermal fibroblasts as described in the text. Lane 1, molecular weight marker; lane 2, rBPI50, where the subscript number indicates molecular size); lane 3, culture supernatant from unstimulated human dermal fibroblasts; lane 4, isotypic control staining of cell culture supernatant from unstimulated human dermal fibroblasts with an irrelevant mouse IgG1 antibody. (B) Shown are the results of quantitative BPI detection in culture supernatants from human dermal fibroblasts by capture ELISA. In culture supernatants from resting fibroblasts, BPI was found in concentrations of 1 ng/1 million cells. These levels were increased by 20-fold after IL-4 stimulation but were not changed by stimulation with IL-1β, TNF-α, S. aureus, P. aeruginosa, C. albicans, and 4-HC.
