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CELLULAR IMMUNOLOGY

Role of Macrophage Migration Inhibitory Factor in Otitis Media with Effusion in Adults

Shin Kariya, Mitsuhiro Okano, Katsuya Aoji, Michiya Kosaka, Emiko Chikumoto, Hisashi Hattori, Koji Yuen, Shinji Nishioka, Keiko Nishioka, Kazunori Nishizaki
Shin Kariya
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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Mitsuhiro Okano
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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  • For correspondence: mokano@cc.okayama-u.ac.jp
Katsuya Aoji
2Department of Otolaryngology, Kagawa Prefectural Central Hospital
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Michiya Kosaka
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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Emiko Chikumoto
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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Hisashi Hattori
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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Koji Yuen
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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Shinji Nishioka
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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Keiko Nishioka
3Department of Otolaryngology, Takinomiya Hospital, Kagawa, Japan
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Kazunori Nishizaki
1Department of Otolaryngology—Head and Neck Surgery, Okayama University Medical and Dental School, Okayama
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DOI: 10.1128/CDLI.10.3.417-422.2003
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ABSTRACT

Otitis media with effusion (OME) is one of the most common ear diseases. Bacterial endotoxins and several inflammatory cytokines appear to be involved in the pathogenesis of OME in children; however, little is known of the immunological aspects of the onset of OME in adults. We sought to determine the presence of macrophage migration inhibitory factor (MIF) as well as interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-α), RANTES (regulated upon activation, normal T-cell expressed and presumably secreted), and endotoxin in middle ear effusions (MEEs) from adult patients with OME. In addition, the levels of MIF in MEEs from adults and children were compared. MEE was obtained from 95 adults and 11 children. The levels of MIF, IL-1β, TNF-α, and RANTES were determined by enzyme-linked immunosorbent assay, and the concentrations of endotoxin and total protein were determined by the Endospec assay and bicinchoninic acid assay, respectively. MIF was detected in 97.9% of the MEEs from adults, while endotoxin, IL-1β, TNF-α, and RANTES were detected in 96.8, 12.6, 5.3, and 43.9%, respectively. In addition, the level of MIF was significantly higher than those of endotoxin, IL-1β, and TNF-α. A positive correlation between the levels of MIF and endotoxin was observed. MIF and endotoxin were detected in 81.8 and 72.7%, respectively, of the MEEs from the children. The level of MIF was significantly higher in the children, and conversely that of endotoxin was significantly higher in the adults. These results suggest that the interaction between MIF and endotoxin may promote fluid collection in the middle ear, particularly in adults.

  • Copyright © 2003 American Society for Microbiology
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Role of Macrophage Migration Inhibitory Factor in Otitis Media with Effusion in Adults
Shin Kariya, Mitsuhiro Okano, Katsuya Aoji, Michiya Kosaka, Emiko Chikumoto, Hisashi Hattori, Koji Yuen, Shinji Nishioka, Keiko Nishioka, Kazunori Nishizaki
Clinical and Diagnostic Laboratory Immunology May 2003, 10 (3) 417-422; DOI: 10.1128/CDLI.10.3.417-422.2003

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Role of Macrophage Migration Inhibitory Factor in Otitis Media with Effusion in Adults
Shin Kariya, Mitsuhiro Okano, Katsuya Aoji, Michiya Kosaka, Emiko Chikumoto, Hisashi Hattori, Koji Yuen, Shinji Nishioka, Keiko Nishioka, Kazunori Nishizaki
Clinical and Diagnostic Laboratory Immunology May 2003, 10 (3) 417-422; DOI: 10.1128/CDLI.10.3.417-422.2003
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