Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Clinical and Vaccine Immunology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About CVI
    • For Librarians
    • For Advertisers
    • FAQ
VETERINARY IMMUNOLOGY

Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis-Infected Cattle

Will L. Goff, Terry F. McElwain, Carlos E. Suarez, Wendell C. Johnson, Wendy C. Brown, Junzo Norimine, Donald P. Knowles
Will L. Goff
1Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: wgoff@vetmed.wsu.edu
Terry F. McElwain
2Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carlos E. Suarez
1Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wendell C. Johnson
1Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wendy C. Brown
2Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Junzo Norimine
2Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Donald P. Knowles
1Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/CDLI.10.1.38-43.2003
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Western blot confirming the presence of the BABB75A4 epitope within the C-terminal RAP-1 recombinant construct rRCT. (A) MAb BABB75A4 was used as the probe. (B) MAb 23.53.156 was used as the probe. Lanes: 1, full-length rRAP-1 fusion protein; 2, rRCT fusion protein; 3, rRNT fusion protein.

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    ELISA block titration with the indicated concentrations of rRCT and MAb BABB75A4.

  • FIG. 3.
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    Effects of temperature and SDS treatment on the stability of rRCT. (A) Purified rRCT dried onto plates and stored for 48 h at the indicated temperatures before reaction with MAb BABB75A4. (B) Purified rRCT dried onto plates and used in a competitive format with MAb BABB75A4 and a known positive control bovine serum immediately (Fresh Antigen), stored at 50°C for 1 month without SDS treatment, and stored at 50°C for 1 month after SDS treatment.

  • FIG. 4.
    • Open in new tab
    • Download powerpoint
    FIG. 4.

    Frequency distribution of percent inhibition of MAb BABB75A4 binding in the cELISA by samples from known B. bovis-infected and uninfected animals.

  • FIG. 5.
    • Open in new tab
    • Download powerpoint
    FIG. 5.

    Kinetics of antibody detection by cELISA. (A) Samples obtained from a group of four cattle before and daily through 23 days after experimental intravenous inoculation of the T2Bo isolate of B. bovis. (B) Samples obtained from a group of five cattle before and every other day beginning on day 13 and continuing through day 98 after experimental intravenous inoculation of the T2Bo isolate of B. bovis. The horizontal dashed line indicates the threshold inhibition of 40%, above which samples are considered positive.

Tables

  • Figures
  • TABLE 1.

    cELISA comparison of B. bovis-infected and uninfected cattle sera

    RCT cELISAb resultNo. of B. bovis samplesaTotal samples
    InfectedUninfected
    Positive1033106
    Negative0224224
    Total103227330
    • ↵ a Samples were from infected animals confirmed microscopically or by nested PCR and from uninfected animals originating and maintained in a Babesia-free region of the northern United States.

    • ↵ b Threshold inhibition of 40%.

  • TABLE 2.

    Concordance of the B. bovis cELISA with the B. bovis IIF analysis

    cELISAb resultIIF analysisTotal samplesa
    No. positiveNo. negative
    Positive44145
    Negative18485
    Total4585130
    • ↵ a Field samples were obtained from Argentina, Bolivia, Morocco, and Puerto Rico.

    • ↵ b Threshold inhibition of 40%.

PreviousNext
Back to top
Download PDF
Citation Tools
Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis-Infected Cattle
Will L. Goff, Terry F. McElwain, Carlos E. Suarez, Wendell C. Johnson, Wendy C. Brown, Junzo Norimine, Donald P. Knowles
Clinical and Diagnostic Laboratory Immunology Jan 2003, 10 (1) 38-43; DOI: 10.1128/CDLI.10.1.38-43.2003

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Clinical and Vaccine Immunology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis-Infected Cattle
(Your Name) has forwarded a page to you from Clinical and Vaccine Immunology
(Your Name) thought you would be interested in this article in Clinical and Vaccine Immunology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis-Infected Cattle
Will L. Goff, Terry F. McElwain, Carlos E. Suarez, Wendell C. Johnson, Wendy C. Brown, Junzo Norimine, Donald P. Knowles
Clinical and Diagnostic Laboratory Immunology Jan 2003, 10 (1) 38-43; DOI: 10.1128/CDLI.10.1.38-43.2003
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About CVI
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1556-6811; Online ISSN: 1556-679X