Article Figures & Data
Tables
- TABLE 1.
Analysis of clinical parameters and P-gp expression in total PBMC, CD4+ T lymphocytes, and CD14+ monocytes from HIV+ patientsa
No. of group Patient code Therapy Viremia (copies/ml) No. of CD4 cells/mm3 Treatment protocol P-gp expression PBMC CD4+ CD14+ 1 CM0612 Naive 2,900 322 1.05 1.23 2.00 GR1411 Naive 2,400 629 1.10 1.21 6.21 AS1512 Naive 2,300 433 0.99 1.06 1.75 VC1512 Naive 190,000 267 1.01 1.22 1.74 BG2211 Naive 25,000 630 1.03 1.00 1.65 2 DF0612 Responder <80 657 2 NRTI + 1 NNRTI 0.99 0.96 1.45 D10612 Responder <80 719 2 NRTI + PI 1.05 1.13 1.22 MC0612 Responder <80 808 2 NRTI + PI 1.08 1.00 1.95 ER1411 Responder <80 356 2 NRTI + 1 NNRTI 0.88 0.88 3.55 AG1512 Responder <80 420 2 NRTI + PI 1.03 1.27 1.34 GW1512 Responder <80 867 2 NRTI + PI 1.00 0.99 1.15 BA2211 Responder <80 805 2 NRTI + 1 NNRTI 1.03 0.96 2.74 OA2211 Responder <80 309 2 NRTI + PI 0.97 0.96 2.74 3 AG0612 Nonresponder 12,000 363 2 NRTI + PI 1.07 1.07 2.02 FL1512 Nonresponder 84,000 20 2 NRTI + PI 0.53 0.57 1.38 PE1512 Nonresponder 13,000 373 2 NRTI + PI 1.01 0.98 1.55 DT2211 Nonresponder 46,000 299 2 NRTI + PI 0.98 1.17 3.31 CF0612 Nonresponder 98,000 64 2 NRTI + 1 NNRTI 1.00 1.11 1.49 ↵ a Cytometric analysis of surface antigen expression was performed as previously described (12) by using the following monoclonal antibodies: anti-CD14-phycoerythrin (clone M5E2; Becton Dickinson, Mountain View, Calif.), anti-CD4-phycoerythrin-Cy5 (clone RPA-T4; Becton Dickinson), and anti-P-gp-fluorescein isothiocyanate (clone 12.10), which was kindly provided by Dr. M. Cianfriglia (Rome, Italy). Briefly, 5 × 105 PBMC were washed in 1% phosphato-buffered saline, 1% bovine serum albumin, and 0.1% sodium azide and were incubated for 15 min at 4°C with monoclonal antibodies. Samples were fixed in 1% paraformaldehyde, immediately acquired by a FACScalibur flow cytometer (Becton Dickinson), and analyzed with CellQuest software (Becton Dickinson). P-gp expression was analyzed in different cellular subsets: total PBMC, CD4+ T lymphocytes, and CD14+ monocytes. Data are indicated as an intensity index, which is obtained by dividing the median fluorescence intensity seen with the P-gp-specific monoclonal antibody by that seen with the immunoglobulin G2a isotype control (p170/immunoglobulin G), as previously described (10). NRTI, nucleoside reverse transcriptase inhibitors; NNRTI, nonnucleoside reverse transcriptase inhibitor.