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Letter to the Editor

P-Glycoprotein Expression by Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Patients Is Independent from Response to Highly Active Antiretroviral Therapy

Chiara Agrati, Fabrizio Poccia, Simone Topino, Pasquale Narciso, Cinzia Selva, Leopoldo Paolo Pucillo, Gianpiero D'Offizi, Guido Antonelli, Francesca Bellomi, Ombretta Turriziani, Federica Bambacioni
Chiara Agrati
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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Fabrizio Poccia
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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Simone Topino
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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Pasquale Narciso
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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Cinzia Selva
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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Leopoldo Paolo Pucillo
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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Gianpiero D'Offizi
National Institute for Infectious Disease  “L. Spallanzani”—IRCCS Rome, Italy
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  • For correspondence: gdoffizi@inmi.it
Guido Antonelli
Department of Experimental Medicine  and Pathology Virology Section University of Rome La Sapienza Rome, Italy
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Francesca Bellomi
Department of Experimental Medicine  and Pathology Virology Section University of Rome La Sapienza Rome, Italy
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Ombretta Turriziani
Department of Experimental Medicine  and Pathology Virology Section University of Rome La Sapienza Rome, Italy
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Federica Bambacioni
Libera Università “Campus Biomedico” Rome, Italy
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DOI: 10.1128/CDLI.10.1.191-192.2003
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  • TABLE 1.

    Analysis of clinical parameters and P-gp expression in total PBMC, CD4+ T lymphocytes, and CD14+ monocytes from HIV+ patientsa

    No. of groupPatient codeTherapyViremia (copies/ml)No. of CD4 cells/mm3Treatment protocolP-gp expression
    PBMCCD4+CD14+
    1CM0612Naive2,9003221.051.232.00
    GR1411Naive2,4006291.101.216.21
    AS1512Naive2,3004330.991.061.75
    VC1512Naive190,0002671.011.221.74
    BG2211Naive25,0006301.031.001.65
    2DF0612Responder<806572 NRTI + 1 NNRTI0.990.961.45
    D10612Responder<807192 NRTI + PI1.051.131.22
    MC0612Responder<808082 NRTI + PI1.081.001.95
    ER1411Responder<803562 NRTI + 1 NNRTI0.880.883.55
    AG1512Responder<804202 NRTI + PI1.031.271.34
    GW1512Responder<808672 NRTI + PI1.000.991.15
    BA2211Responder<808052 NRTI + 1 NNRTI1.030.962.74
    OA2211Responder<803092 NRTI + PI0.970.962.74
    3AG0612Nonresponder12,0003632 NRTI + PI1.071.072.02
    FL1512Nonresponder84,000202 NRTI + PI0.530.571.38
    PE1512Nonresponder13,0003732 NRTI + PI1.010.981.55
    DT2211Nonresponder46,0002992 NRTI + PI0.981.173.31
    CF0612Nonresponder98,000642 NRTI + 1 NNRTI1.001.111.49
    • ↵ a Cytometric analysis of surface antigen expression was performed as previously described (12) by using the following monoclonal antibodies: anti-CD14-phycoerythrin (clone M5E2; Becton Dickinson, Mountain View, Calif.), anti-CD4-phycoerythrin-Cy5 (clone RPA-T4; Becton Dickinson), and anti-P-gp-fluorescein isothiocyanate (clone 12.10), which was kindly provided by Dr. M. Cianfriglia (Rome, Italy). Briefly, 5 × 105 PBMC were washed in 1% phosphato-buffered saline, 1% bovine serum albumin, and 0.1% sodium azide and were incubated for 15 min at 4°C with monoclonal antibodies. Samples were fixed in 1% paraformaldehyde, immediately acquired by a FACScalibur flow cytometer (Becton Dickinson), and analyzed with CellQuest software (Becton Dickinson). P-gp expression was analyzed in different cellular subsets: total PBMC, CD4+ T lymphocytes, and CD14+ monocytes. Data are indicated as an intensity index, which is obtained by dividing the median fluorescence intensity seen with the P-gp-specific monoclonal antibody by that seen with the immunoglobulin G2a isotype control (p170/immunoglobulin G), as previously described (10). NRTI, nucleoside reverse transcriptase inhibitors; NNRTI, nonnucleoside reverse transcriptase inhibitor.

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P-Glycoprotein Expression by Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Patients Is Independent from Response to Highly Active Antiretroviral Therapy
Chiara Agrati, Fabrizio Poccia, Simone Topino, Pasquale Narciso, Cinzia Selva, Leopoldo Paolo Pucillo, Gianpiero D'Offizi, Guido Antonelli, Francesca Bellomi, Ombretta Turriziani, Federica Bambacioni
Clinical and Diagnostic Laboratory Immunology Jan 2003, 10 (1) 191-192; DOI: 10.1128/CDLI.10.1.191-192.2003

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P-Glycoprotein Expression by Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Patients Is Independent from Response to Highly Active Antiretroviral Therapy
Chiara Agrati, Fabrizio Poccia, Simone Topino, Pasquale Narciso, Cinzia Selva, Leopoldo Paolo Pucillo, Gianpiero D'Offizi, Guido Antonelli, Francesca Bellomi, Ombretta Turriziani, Federica Bambacioni
Clinical and Diagnostic Laboratory Immunology Jan 2003, 10 (1) 191-192; DOI: 10.1128/CDLI.10.1.191-192.2003
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