CVI Accepts, published online ahead of print on 5 November 2008

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Clin. Vaccine Immunol. doi:10.1128/CVI.00304-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Antibodies

Shashidhara Y. Marulappa and Sanjay Kapil^*

Oklahoma Animal Disease Diagnostic Laboratory (OADDL), Center for Veterinary Health Sciences, Stillwater, OK 74078

^* To whom correspondence should be addressed. Email: sanjay.kapil{at}okstate.edu.

Canine parvovirus (CPV) is the number one viral cause of enteritis, morbidity and mortality in 8-week old young puppies. We have developed twin assays (slide agglutination test for CPV antigen/slide inhibition test for CPV antibody, SAT/SIT) that are sensitive, specific, cost effective, generic for all genotypes of CPV, and provide instant results for CPV antigen detection in feces and antibody quantification in serum. We found these assays to be useful for routine applications in kennels with large numbers of puppies at risk. The results of these assays are available in 1 minute and do not require any special instrumentation. SAT/SIT technology will find applications in rapid screening of samples for other hemagglutination emerging viruses of animals and humans (influenza and SARS coronavirus).