CVI Accepts, published online ahead of print on 25 July 2007

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Clin. Vaccine Immunol. doi:10.1128/CVI.00187-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

AN APPROACH TO PERIPHERAL BLOOD MONONONUCLEAR CELL CRYOPRESERVATION QUALITY ASSURANCE PROGRAM

Adriana Weinberg^*, Raul Louzao, Marisa M. Mussi-Pinhata, Maria L. S. Cruz, Jorge A. Pinto, Maria F. Huff, Andrea C. de Castro, Maria C. Sucupira, and Thomas W. Denny

University of Colorado Health Sciences Center, Denver, CO; Duke University, Raleigh-Durham, NC; Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Hospital do Servidor Estadual, Rio de Janeiro, RJ, Brazil, Universidade de Minas Gerais, Belo Horizonte, MG, Brazil, Universidade Federal de Rio de Janeiro, Rio de Janeiro, RJ, Brazil; Hospital da Conceicão, Porto Alegre, RS, Brazil; Universidade Federal de São Paulo, São Paulo, SP, Brazil

^* To whom correspondence should be addressed. Email: Adriana.Weinberg{at}uchsc.edu.

Seven Brazilian sites participating in the Pediatric ACTG international cryopreservation quality assurance pilot, cryopreserved and shipped PBMC for analysis to a central US laboratory. The cell viability and recovery significantly increased over time. A wet-lab training session conducted at the central laboratory significantly improved the quality of the cryopreserved PBMC.