CVI Accepts, published online ahead of print on 18 July 2007

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cook, E. Articles by Fries, B. C. Search for Related Content PubMed PubMed Citation Articles by Cook, E. Articles by Fries, B. C.

 Previous Article  |  Next Article 

Clin. Vaccine Immunol. doi:10.1128/CVI.00183-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Measurement of Staphylococcal enterotoxin B in serum and culture supernatant with a Capture Elisa

E. Cook, X. Wang, N. Robiou, and B. C. Fries

Departments of Medicine, Microbiology and Immunology at Albert Einstein College of Medicine Bronx New York Fordham University Bronx, New York

Staphylococcal enterotoxin B (SEB) is a select agent because it is a potent mitogen that elicits life-threatening polyclonal T-cell proliferation and cytokine production at very low concentrations. Efforts are in progress to develop therapeutic reagents and vaccines that neutralize or prevent the devastating effects of this toxin. Because of rapid binding to in vivo receptors, this toxin is difficult to detect in serum. This rapid binding also constitutes a major challenge for the development of effective therapeutic reagents that can neutralize the effects of the toxin in vivo. We have developed a highly sensitive capture ELISA that detects SEB in body fluids at very low levels. With this assay, peak serum levels of SEB and renal clearance can be measured in mice. After either oral ingestion or nasal inhalation of SEB by mice, this assay documents transcytosis of SEB across the mucosal membranes into serum within 2 hours. Furthermore, this assay was used to compare SEB levels in different murine models for SEB induced lethal shock and demonstrated that co-administration of toxin enhancing chemicals such as D-galactosamine and LPS can alter peak serum levels of SEB. Hence, this assay is a potentially useful tool for the study of SEB pharmacokinetics and the effects of potential therapeutic reagents on SEB serum levels.

This article has been cited by other articles:

• Varshney, A. K., Mediavilla, J. R., Robiou, N., Guh, A., Wang, X., Gialanella, P., Levi, M. H., Kreiswirth, B. N., Fries, B. C. (2009). Diverse Enterotoxin Gene Profiles among Clonal Complexes of Staphylococcus aureus Isolates from the Bronx, New York. Appl. Environ. Microbiol. 75: 6839-6849 [Abstract] [Full Text]