CVI Accepts, published online ahead of print on 18 June 2008
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Clin. Vaccine Immunol. doi:10.1128/CVI.00157-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Development of a more efficient algorithm for identifying false-positive reactivity in a dengue IgM screening assay

Harry E. Prince*, Cindy Yeh, and Mary Lapé-Nixon

Focus Diagnostics Inc., Cypress, CA

* To whom correspondence should be addressed. Email: hprince{at}focusdx.com.


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Abstract

Of 2692 sera screened for dengue IgM using a mu-capture enzyme-linked immunosorbent assay (ELISA), 954 were equivocal (index 0.90-1.10) or positive (index >1.10), and retested using a background subtraction (BS) ELISA that identifies screen false-positives. No false-positives were found among 427 sera with screen-ELISA indices >6.00; thus, retesting this specimen subset by BS-ELISA is unnecessary.






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