This Article Full Text (PDF) Other Versions of this Article: CVI.00136-06v1 14/1/99    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yip, C. W. Articles by Leung, F. C. Search for Related Content PubMed PubMed Citation Articles by Yip, C. W. Articles by Leung, F. C.

 Previous Article  |  Next Article 

Clin. Vaccine Immunol. doi:10.1128/CVI.00136-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Analytical interferences of naturally occurring anti-E. coli proteins antibodies in the serum of healthy human to recombinant nucleocapsid protein-based ELISA for serodiagnosis of SARS

Department of Zoology and Department of Microbiology, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China

^* To whom correspondence should be addressed. Email: fcleung{at}hkucc.hku.hk.

	Abstract

We reported the analytical interference of anti-E. coli proteins (EP) antibodies in human sera and residual EP in recombinant nucleocapsid protein-based ELISA as a possible source of false positives in SARS serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.