CVI Accepts, published online ahead of print on 25 July 2007

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Clin. Vaccine Immunol. doi:10.1128/CVI.00092-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Distribution and Evolution of T Cell Receptor Vbeta Repertoire on Peripheral Blood Lymphocytes of Newborn Infants of HIV-Infected Mothers: Differential Display on CD4 and CD8 T cells and Effect of HIV Infection

William Borkowsky^*, Song-He Chen, and Ilana Belitskaya-Levy

The Departments of Pediatrics and Biostatistics, The New York University School of Medicine, 550 First Avenue, New York NY 10016

Neonatal human peripheral blood mononuclear cells from 12 HIV-infected and 84 uninfected children were assessed for their distribution of T cell receptors by flow cytometry employing monoclonal antibodies to 14 types. Vbeta 2, 5c, or 13 were the most commonly found on CD4 cells, in that order. There was a bimodal distribution of Vbeta 2, being most common in 48% of individuals but in limiting frequency (<2% of CD4) in 21%. Vbeta 2, 3, 8b and 13 were most commonly expressed on CD8 cells in similar frequencies. There was little difference in the pattern displayed among the infected compared to the uninfected. The variation of the distribution over time was studied in 12 infants (7 infected). Only a single HIV-infected child had a significant difference in the interquartile range; none of the HIV negative patients showed a significant difference. In conclusion, newborns demonstrate different distribution of T cell receptor (TCR) V beta types on CD4 and CD8 cells. HIV infection produces no change in neonatal TCR and little change over the course of two years compared to that seen in the uninfected.