This Article Full Text (PDF) Other Versions of this Article: CVI.00057-06v1 14/1/81    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hotta, A. Articles by Yamada, A. Search for Related Content PubMed PubMed Citation Articles by Hotta, A. Articles by Yamada, A.

 Previous Article  |  Next Article 

Clin. Vaccine Immunol. doi:10.1128/CVI.00057-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Preparation of Monoclonal Antibodies for Detection and Identification of Francisella tularensis

Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan

^* To whom correspondence should be addressed. Email: ahotta{at}nih.go.jp,

	Abstract

Monoclonal antibodies (MAbs) against Francisella tularensis were obtained. Three MAbs specifically reacted with F. tularensis while 4 MAbs reacted with other members of the genus Francisella as well. Fluorescent isothiocyanateconjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinated F. tularensis antigen in the agglutination tests. These MAbs should improve detection and identification methods of F. tularensis.