CVI Accepts, published online ahead of print on 19 March 2008
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Clin. Vaccine Immunol. doi:10.1128/CVI.00029-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Diagnosis of Tuberculous Meningitis due to ESAT-6-specific IFN-gamma production detected by Enzyme-Linked Immunospot Assay in Cerebrospinal Fluid

Shuji Murakami, Mitsuhiro Takeno, Hideaki Oka, Atsuhisa Ueda, Takashi Kurokawa, Yoshiyuki Kuroiwa, and Yoshiaki Ishigatsubo*

Department of Internal Medicine and Clinical Immunology, and Department of Neurology, Yokohama City University Graduate School of Medicine, Yokohama, Japan

* To whom correspondence should be addressed. Email: ishigats{at}med.yokohama-cu.ac.jp.


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Abstract

A coma patient was diagnosed as having tuberculous meningitis by detection of ESAT-6 specific IFN-{gamma} secreting cells within cerebrospinal fluid by enzyme-linked immunospot assay prior to identifying the pathogen in the cerebrospinal fluid culture.






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