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Clin. Vaccine Immunol. doi:10.1128/CVI.00011-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

RAPID DIAGNOSIS OF BURKHOLDERIA PSEUDOMALLEI INFECTION IN PATIENTS WITH COMMUNITY- ACQUIRED SEPTICEMIA BY USING PROTEIN- SPECIFIC MONOCLONAL ANTIBODY- BASED LATEX AGGLUTINATION

Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Department of Microbiology, Faculty of Medicine, Srinakarinwirot University, Bangkok, National Institute of Health, Department of Medical Services, Ministry of Public Health, Nonthaburi, Thailand

^* To whom correspondence should be addressed. Email: sipep{at}mahidol.ac.th.

	Abstract

A latex agglutination test employing monoclonal antibody specific to 30 kDa protein of Burkholderia pseudomallei was used to detect the organisms in blood culture broth from 1,139 patients with community acquired septicemia. The sensitivity, specificity, positive and negative predictive value of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.