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Clinical and Diagnostic Laboratory Immunology, September 1999, p. 701-704, Vol. 6, No. 5
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Reverse-Sandwich Enzyme-Linked Immunosorbent Assay for Verocytotoxin 1 and 2 Antibodies in Human and Bovine Sera

H. Miyazawa,1,* H. Bannai,1 T. Yanase,2 C. Morita,2 S. Satoh,3 J. Sugiyama,3 S. Taniguchi,4 and S. Inouye4

Department of Medical Technology, Kyorin University School of Health Sciences, Hachioji-shi,1 and Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Shinjuku-ku,4 Tokyo, Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu-shi, Hokkaido,2 and Denka Seiken Co., Gosen-shi, Niigata,3 Japan

Received 16 February 1999/Returned for modification 4 May 1999/Accepted 25 June 1999

A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and animal sera. This assay has two advantages over a conventional indirect ELISA: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. The VT1 (Shiga-like toxin 1) antibody-positive rates were 5% in 202 normal adult humans and 99% in 93 normal cattle at a dairy farm. This ELISA is most suitable for seroepidemiologic studies of infections with VT-producing Escherichia coli in humans and various animal species.

* Corresponding author. Mailing address: Department of Medical Technology, Kyorin University School of Health Sciences, 476 Miyashita-cho, Hachioji-shi, Tokyo 192-8508, Japan. Phone: 81-426-91-0011. Fax: 81-426-91-1094. E-mail: hiro_mzw{at}d1.dion.ne.jp.

Clinical and Diagnostic Laboratory Immunology, September 1999, p. 701-704, Vol. 6, No. 5
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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