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Clinical and Vaccine Immunology, May 2009, p. 733-738, Vol. 16, No. 5
1071-412X/09/$08.00+0     doi:10.1128/CVI.00493-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Molecular Genetic Characterization of the Merozoite Surface Protein 1 Gene of Plasmodium vivax from Reemerging Korean Isolates{triangledown}

So-Hee Kim,1 Seung-Young Hwang,2 Jeong Hwan Shin,3,4 Chi-Sook Moon,5 Dong-Wook Kim,5 and Weon-Gyu Kho1,2,6*

Department of Malariology,1 Paik Institute for Clinical Research,3 Department of Parasitology,2 Department of Laboratory Medicine,4 Department of Internal Medicine,5 Mitochondrial Research Group (Frontier Inje Research for Science and Technology) Inje University College of Medicine, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, South Korea6

Received 31 December 2008/ Accepted 25 February 2009

Plasmodium vivax merozoite surface protein 1 (PvMSP-1) has been considered a major candidate for the development of an antimalaria vaccine, but the molecule exhibits antigenic diversity among isolates. The extent of genetic polymorphism in the region between interspecies conserved blocks 4 and 5 (ICB4 and ICB5) of the PvMSP-1 gene was analyzed for 30 Korean isolates. Two genotypes, SK-A and SK-B, were identified on the basis of amino acid substitution. Almost all the amino acid sequences of the Korean isolates were nearly identical to those of the Solomon Island isolate Solo-83 (97.8 to 99.9% similarity) and Philippine isolates Ph-79, Ph-52-2, and Ph-49 (97.3 to 99.8% similarity). Also, we report two sequences in the isolates that were characterized on the basis of restriction fragment length polymorphism (RFLP). The RFLP profiles following digestion with the DraI restriction enzyme produced two distinguishable patterns. This study might be the first report of the region between ICB4 and ICB5 of the MSP-1 gene of P. vivax in South Korea.

* Corresponding author. Mailing address: Department of Malariology, Paik Institute for Clinical Research, and Department of Parasitology, Inje University College of Medicine, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, South Korea. Phone: 82-51-890-6731. Fax: 82-51-894-6709. E-mail: wgkho{at}inje.ac.kr

{triangledown} Published ahead of print on 4 March 2009.

Clinical and Vaccine Immunology, May 2009, p. 733-738, Vol. 16, No. 5
1071-412X/09/$08.00+0     doi:10.1128/CVI.00493-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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