This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qiu, L.-w.
Right arrow Articles by Che, X.-y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qiu, L.-w.
Right arrow Articles by Che, X.-y.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, January 2009, p. 88-95, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00212-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus Serotype 2 Infections{triangledown}

Li-wen Qiu,1,3,§ Biao Di,2,§ Kun Wen,1,3 Xin-shuai Wang,1 Wei-hua Liang,3 Ya-di Wang,1,3 Yu-xian Pan,1,3 Ming Wang,2 Yan-qing Ding,4 and Xiao-yan Che1,3*

Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China,1 Center for Disease Control and Prevention of Guangzhou, Guangzhou, China,2 Clinical Immunology Center, Southern Medical University, Guangzhou, China,3 Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China4

Received 17 May 2008/ Returned for modification 28 July 2008/ Accepted 3 November 2008

The dengue virus (DENV) has four distinct serotypes (DENV1, DENV2, DENV3, and DENV4) that require differentiation for effective prevention of morbid diseases. The recently developed DENV1-specific NS1 antigen capture enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies (MAbs) that recognize distinct epitopes on nonstructural protein 1 (NS1) of a specific DENV serotype is convenient and cost-effective, but assays have not yet been developed for DENV serotypes 2 to 4. This paper describes the development and validation of a DENV2-specific NS1 antigen capture ELISA by selection and optimization of the pair of well-characterized MAbs that recognized epitopes specific for DENV2 NS1 from a large panel of MAbs. The DENV2 NS1 ELISA displayed exclusive sensitivity with the DENV2 serotype and did not cross-react with the other three DENV serotypes. The sensitivity and specificity of the DENV2 NS1 ELISA were 83.3% (25/30) and 100% (504/504) when used to test 30 acute-phase serum samples from patients infected with DENV2 identified by virus isolation or reverse transcription-PCR serotyping and 504 serum samples from healthy individuals, respectively. The specificity of this assay was also evaluated using a panel of serum samples which were positive for DENV1, other flaviviruses, and nonflaviviruses; no cross-reactions were observed in these clinical samples. The DENV2 NS1 ELISA was eightfold more sensitive than a commercially available serotype-cross-reactive NS1 ELISA (Panbio Diagnostics, Brisbane, Australia) when the two assays were used to test the DENV2-infected cell culture supernatants in parallel. The Panbio NS1 ELISA displayed variation in sensitivity between DENV serotypes. The DENV2-specific NS1 antigen capture ELISA can be used as a tool for the rapid identification of DENV2 infections.

* Corresponding author. Mailing address: Clinical Laboratory, Zhujiang Hospital, Southern Medical University, No. 253 Gong ye da dao zhong, Guangzhou 510282, China. Phone and fax: 86-20-61643592. E-mail: linche{at}pub.guangzhou.gd.cn

{triangledown} Published ahead of print on 19 November 2008.

§ L.Q. and B.D. contributed equally to this work.

Clinical and Vaccine Immunology, January 2009, p. 88-95, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00212-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»