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Clinical and Vaccine Immunology, January 2009, p. 78-87, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00253-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Influence of Promoter, Gene Copy Number, and Preexisting Immunity on Humoral and Cellular Responses to a Vectored Antigen Delivered by a Salmonella enterica Vaccine{triangledown}

Manvendra Saxena, Peter J. Coloe, and Peter M. Smooker*

School of Applied Sciences, RMIT University, Bundoora, Victoria, Australia

Received 12 July 2008/ Returned for modification 8 October 2008/ Accepted 30 October 2008

Attenuated Salmonella strains are currently in production as vaccines for protection of animals against salmonellosis. Such commercial strains offer the potential to deliver heterologous antigen to protect animals against other diseases. One vaccine strain, attenuated Salmonella enterica serovar Typhimurium (STM-1), was tested for the ability to deliver ovalbumin and to induce immune responses in mice. Two vaccine trials were performed testing the influence of promoter choice, the location of the encoding DNA (plasmid or chromosome), and the effect of preexisting homologous or heterologous immunity. The results demonstrated that humoral and T-cell responses were induced from either of two promoters, from either the plasmid or the chromosome, and that preexposure to the empty homologous vector, STM-1, or the heterologous vector, S. enterica serovar Enteritidis, had no detrimental effect on subsequent antigen-specific responses. In the case of homologous preexposure, responses were generally greater, and this was correlated with an increased uptake of Salmonella by macrophages in vitro after opsonization with immune sera.

* Corresponding author. Mailing address: School of Applied Sciences, RMIT University, P.O. Box 71, Bundoora, VIC, 3083, Australia. Phone: (613) 99257129. Fax: (613) 99257110. E-mail: peter.smooker{at}rmit.edu.au

{triangledown} Published ahead of print on 12 November 2008.

Clinical and Vaccine Immunology, January 2009, p. 78-87, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00253-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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