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Clinical and Vaccine Immunology, January 2009, p. 66-72, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00346-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection of Antibodies against Epidermodysplasia Verruciformis-Associated Canine Papillomavirus 3 in Sera of Dogs from Europe and Africa by Enzyme-Linked Immunosorbent Assay ^,

C. E. Lange,^1,2 K. Tobler,¹ C. Favrot,² M. Müller,³ J. O. Nöthling,⁴ and M. Ackermann^1*

Institute of Virology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland,¹ Clinic for Small Animal Internal Medicine, Dermatology Unit, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland,² Applied Tumor Virology, Deutsches Krebsforschungszentrum, Heidelberg, Germany,³ Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa⁴

Received 17 September 2008/ Returned for modification 10 November 2008/ Accepted 17 November 2008

The role of papillomaviruses (PVs) in the development of canine cancers is controversial. However, recently a novel canine PV (CPV3) was detected in a dog affected with a condition reminiscent of epidermodysplasia verruciformis (EV). The aim of the present study was to investigate the seroprevalence of CPV3 by using generic enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against either canine oral PV (COPV) or CPV3. Therefore, the capsid proteins of both PV types were expressed as glutathione S-transferase fusion protein antigens and adsorbed to glutathione-casein-coated ELISA plates. After showing that PV type-specific antibodies could be detected in the sera from dogs with confirmed COPV or CPV3 infection, CPV3- and COPV-seropositive samples were detected in two sets of canine sera collected in Switzerland and South Africa, respectively. We found specific antibodies against COPV and CPV3 among the tested sera and also a large number that were positive for both antigens. The seroprevalences of PV antibodies of 21.9% (COPV) and 26.9% (CPV3) among the tested dogs from South Africa were higher than those among the dogs from Switzerland at 10.5% (COPV) and 1.3% (CPV3). Our data suggest a need for further CPV-related seroepidemiological surveys in different countries, especially in the context of clinical manifestations and possible breed predispositions. For this purpose, the newly developed ELISAs can be a useful tool.

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* Corresponding author. Mailing address: Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland. Phone: 41 44 635 87 01. Fax: 41 44 635 89 11. E-mail: email{at}vetvir.uzh.ch.

Published ahead of print on 26 November 2008.

Supplemental material for this article may be found at http://cvi.asm.org/.

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Clinical and Vaccine Immunology, January 2009, p. 66-72, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00346-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Lange, C. E., Tobler, K., Ackermann, M., Panakova, L., Thoday, K. L., Favrot, C. (2009). Three novel canine papillomaviruses support taxonomic clade formation. J. Gen. Virol. 90: 2615-2621 [Abstract] [Full Text]