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Clinical and Vaccine Immunology, January 2009, p. 55-60, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00351-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays{triangledown}

Thomas B. Martins,1,{dagger}* Ryan J. Welch,1,{dagger}* Harry R. Hill,1,2,3,4 and Christine M. Litwin1,2

Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah,1 Departments of Pathology,2 Pediatrics,3 Medicine, University of Utah School of Medicine, Salt Lake City, Utah4

Received 19 September 2008/ Returned for modification 4 November 2008/ Accepted 13 November 2008

The human herpes simplex virus (HSV) is highly pathogenic, with infections caused by two distinct antigenic types, HSV-1 and HSV-2. Differentiation of antibodies to these specific antigens can provide useful information for the diagnosis of subclinical or undiagnosed HSV-2 infections, as well as for reducing the risk of maternal transfer of HSV to the neonate. In this study, a multiplex assay capable of concurrent detection of HSV-1 and -2 immunoglobulin G (IgG) antibodies was compared to immunoblot, Western blot, and enzyme-linked immunosorbent assays. Agreement of the multiplex assay was 95% or greater (n = 332) for both HSV-1 and -2 compared to the three assays. Sensitivities for HSV-1 ranged from 94.9 to 97.9%, with specificities of 93 to 97%. For HSV-2, the sensitivity and specificity ranges were 92.6 to 98.9% and 98.3 to 98.7%, respectively. Our studies show that the multiplexed microsphere-based assay offers a sensitive and specific alternative method for the detection HSV-1 and -2 type-specific antibodies. Advantages of the multiplex assay include multiple results per assay, the inclusion of internal controls for each specimen, and higher throughput of results.

* Corresponding author. Mailing address: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787. Fax: (801) 584-5109. E-mail for T. Martins: martintb{at}aruplab.com. E-mail for R. Welch: ryan.welch{at}aruplab.com

{triangledown} Published ahead of print on 19 November 2008.

{dagger} T.B.M. and R.J.W. made equal contributions to this study.

Clinical and Vaccine Immunology, January 2009, p. 55-60, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00351-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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