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Clinical and Vaccine Immunology, January 2009, p. 49-54, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00334-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Peptide-Based Antibody Detection for Tuberculosis Diagnosis{triangledown}

Guomiao Shen,1 Digambar Behera,2 Manpreet Bhalla,2 Arthur Nadas,3 and Suman Laal1,4,5*

Department of Pathology, New York University School of Medicine, New York, New York 10016,1 Lala Ram Sarup Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India,2 Department of Environmental Medicine, New York University School of Medicine, New York, New York 10016,3 Department of Microbiology, New York University School of Medicine, New York, New York 10016,4 New York Harbor Health Care System, New York, New York 100105

Received 1 August 2008/ Returned for modification 11 October 2008/ Accepted 3 November 2008

Tuberculosis (TB) is a major cause of morbidity and mortality, especially in developing countries. Despite significant limitations, microscopy remains the cornerstone of the global TB control strategy. As the TB epidemic escalates, new diagnostic methods that are accurate and also economical and simple to manufacture and deploy are urgently needed. Although several promising antigens have been identified and evaluated in recent years, the reproducible production of high-quality recombinant mycobacterial proteins with minimal batch-to-batch variation is difficult, laborious, and expensive. To determine the feasibility of devising a synthetic peptide-based diagnostic test for TB, we have delineated the immunodominant epitopes of three candidate antigens, Ag85B, BfrB, and TrxC, that were previously identified to be immunogenic in TB patients. The results demonstrate that combinations of carefully selected synthetic peptides derived from highly immunogenic proteins can be the basis for devising an immunodiagnostic test for TB.

* Corresponding author. Mailing address: Department of Pathology, New York University School of Medicine, 423 East 23rd St., Room, 18123 North, New York, NY 10010. Phone: (212) 263-4164. Fax: (212) 951-6321. E-mail: suman.laal{at}med.nyu.edu

{triangledown} Published ahead of print on 12 November 2008.

Clinical and Vaccine Immunology, January 2009, p. 49-54, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00334-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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