This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sousa, S.
Right arrow Articles by Dardé, M.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sousa, S.
Right arrow Articles by Dardé, M.-L.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, September 2008, p. 1380-1386, Vol. 15, No. 9
1071-412X/08/$08.00+0     doi:10.1128/CVI.00186-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of GRA6-Derived Synthetic Polymorphic Peptides in an Immunoenzymatic Assay To Serotype Toxoplasma gondii in Human Serum Samples Collected from Three Continents{triangledown}

Susana Sousa,1,4 Daniel Ajzenberg,4 Manuel Vilanova,2 José Costa,3 and Marie-Laure Dardé4*

Center of Parasite Immunology and Biology, INSA, Rua de S. Luis 16, 4000-509 Porto, Portugal,1 Instituto de Ciências Biomédicas de Abel Salazar, Largo do Professor Abel Salazar 2, 4099-003, Porto, Portugal,2 Laboratory for Veterinary Research, LNIV-INRB, I.P. Rua dos Lagidos, Lugar da Madalena, 4485-655 Vairão VCD, Portugal,3 Université de Limoges, EA 3174-NETEC, and National Reference Center and Biological resource Center for Toxoplasmosis, CHU Dupuytren, 2 Av. Martin Luther King, 87042 Limoges Cedex, France4

Received 3 May 2008/ Returned for modification 23 June 2008/ Accepted 22 July 2008

Serotyping is a simple typing method that consists of an immunoenzymatic assay (enzyme-linked immunosorbent assay [ELISA]) using synthetic polymorphic peptides derived from Toxoplasma gondii antigens. We developed a new ELISA based on GRA6 C-terminal polymorphic peptides. Serum samples from 41 human infections due to 23 archetypal (type I, II, or III) and 18 nonarchetypal strains were selected in order to validate this approach. For 20 out of the 23 archetypal infections, there was a clear correlation between microsatellite genotype and GRA6 serotyping. All infections due to nonarchetypal strains were misclassified as archetypal strain infections. The GRA6 C-terminal peptides from these strains were analyzed to explain this misclassification. A second group of 455 patients with acute and chronic toxoplasmosis due to unknown genotypes from different European, African, and Latin American countries were included in this study, and the strain type predicted by this method. The results suggest that serotyping is a promising method for typing strains, although limitations exist for African and South American strains as a consequence of higher peptide polymorphism. Other peptides from different markers must be studied in order to discriminate archetypal from nonarchetypal strains.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, CHRU Dupuytren, 2 Av. Martin Luther King, 87042 Limoges Cedex, France. Phone: 33 555 056 160. Fax: 33 555 056 177. E-mail: darde{at}unilim.fr

{triangledown} Published ahead of print on 30 July 2008.

Clinical and Vaccine Immunology, September 2008, p. 1380-1386, Vol. 15, No. 9
1071-412X/08/$08.00+0     doi:10.1128/CVI.00186-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sousa, S., Ajzenberg, D., Marle, M., Aubert, D., Villena, I., da Costa, J. C., Darde, M.-L. (2009). Selection of Polymorphic Peptides from GRA6 and GRA7 Sequences of Toxoplasma gondii Strains To Be Used in Serotyping. CVI 16: 1158-1169 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»