This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Østergaard, E.
Right arrow Articles by Stawski, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Østergaard, E.
Right arrow Articles by Stawski, G.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, August 2008, p. 1244-1247, Vol. 15, No. 8
1071-412X/08/$08.00+0     doi:10.1128/CVI.00155-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Elimination of Interfering Activity in Serum Samples in the Chinese Hamster Ovary Pertussis Serology Assay {triangledown}

Erik Østergaard,{dagger} Charlotte Sørensen,* Lene Nielsen, and Gitte Stawski

Quality Control Department, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark

Received 1 May 2008/ Returned for modification 19 May 2008/ Accepted 12 June 2008

An interfering substance in various frozen serum samples was observed to inhibit the adhesion of Chinese hamster ovary (CHO) cells to microplate surfaces during a CHO pertussis neutralization test, resulting in wells that lacked cells or wells with dead cells after 2 days of incubation. The interfering activity in the serum could be eliminated by (i) transferring cells to other wells after their initial incubation, (ii) adding fetal calf serum (FCS) to the sample dilution buffer, (iii) precoating microplates with FCS, or (iv) preincubating the samples at 4°C for 5 days. Preincubating the samples at 4°C for 5 days reduced the interfering activity in only some of the samples. Adding serum to the sample dilution buffer or precoating the microplates with serum did not influence the antibody titers in the serum samples. The method described may be used for routine applications.

* Corresponding author. Mailing address: Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark. Phone: 45-32683373. Fax: 45-32683872. E-mail: chs{at}ssi.dk

{triangledown} Published ahead of print on 18 June 2008.

{dagger} Present address: Danish Medicines Agency, Axel Heides Gade 1, DK-2300 Copenhagen S, Denmark.

Clinical and Vaccine Immunology, August 2008, p. 1244-1247, Vol. 15, No. 8
1071-412X/08/$08.00+0     doi:10.1128/CVI.00155-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»