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Clinical and Vaccine Immunology, August 2008, p. 1208-1213, Vol. 15, No. 8
1071-412X/08/$08.00+0     doi:10.1128/CVI.00027-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Bovine Colostrum Contains Immunoglobulin G Antibodies against Intimin, EspA, and EspB and Inhibits Hemolytic Activity Mediated by the Type Three Secretion System of Attaching and Effacing Escherichia coli{triangledown}

Daniel A. Vilte,1,{ddagger} Mariano Larzábal,2,{ddagger} Ángel A. Cataldi,2 and Elsa C. Mercado1*

Instituto de Patobiología,1 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Las Cabañas, 1712 Castelar, Prov. Buenos Aires, Argentina2

Received 22 January 2008/ Returned for modification 6 March 2008/ Accepted 9 June 2008

Enterohemorrhagic Escherichia coli (EHEC) is the main cause of hemolytic-uremic syndrome, an endemic disease in Argentina which had an incidence in 2005 of 13.9 cases per 100,000 children younger than 5 years old. Cattle appear to be a major reservoir of EHEC, and a serological response to EHEC antigens has been demonstrated in natural and experimental infections. In the current study, antibodies against proteins implicated in EHEC's ability to form attaching and effacing lesions, some of which are exported to the host cell via a type three secretion system (TTSS), were identified in bovine colostrum by Western blot analysis. Twenty-seven (77.0%) of the 35 samples examined contained immunoglobulin G (IgG) antibodies against the three proteins assayed in this study: EspA, EspB, and the carboxy-terminal 280 amino acids of {gamma}-intimin, an intimin subtype associated mainly with O157:H7 and O145:H- serotypes. Every colostrum sample was able to inhibit, in a range between 45.9 and 96.7%, the TTSS-mediated hemolytic activity of attaching and effacing E. coli. The inhibitory effect was partially mediated by IgG and lactoferrin. In conclusion, we found that early colostrum from cows contains antibodies, lactoferrin, and other unidentified substances that impair TTSS function in attaching and effacing E. coli strains. Bovine colostrum might act by reducing EHEC colonization in newborn calves and could be used as a prophylactic measure to protect non-breast-fed children against EHEC infection in an area of endemicity.

* Corresponding author. Mailing address: Instituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria, CC25- (1712) Castelar, Prov. Buenos Aires, Argentina. Phone: 54 11 4621 1289, ext. 25. Fax: 54 11 4621 1712. E-mail: emercado{at}cnia.inta.gov.ar

{triangledown} Published ahead of print on 18 June 2008.

{ddagger} D.A.V. and M.L. contributed equally to this work.

Clinical and Vaccine Immunology, August 2008, p. 1208-1213, Vol. 15, No. 8
1071-412X/08/$08.00+0     doi:10.1128/CVI.00027-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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