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Clinical and Vaccine Immunology, July 2008, p. 1124-1127, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00481-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of a Multicolor, Single-Tube Technique To Enumerate Lymphocyte Subpopulations{triangledown}

F. Colombo,*{dagger} A. Cattaneo,{dagger} R. Lopa, P. Portararo, P. Rebulla, and L. Porretti

Centre of Transfusion Medicine, Cellular Therapy and Cryobiology, Regenerative Medicine Department, IRCCS Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy

Received 10 December 2007/ Returned for modification 26 February 2008/ Accepted 21 April 2008

To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunophenotyping.

* Corresponding author. Mailing address: Centro di Medicina Trasfusionale, Terapia Cellulare e Criobiologia, IRCCS Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Via F. Sforza 35, 20122 Milan, Italy. Phone: 39/02/55034255. Fax: 39/02/55034183. E-mail: labcitofl{at}policlinico.mi.it

{triangledown} Published ahead of print on 30 April 2008.

{dagger} F.C. and A.C. contributed equally to this work.

Clinical and Vaccine Immunology, July 2008, p. 1124-1127, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00481-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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