This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calderaro, A.
Right arrow Articles by Dettori, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Calderaro, A.
Right arrow Articles by Dettori, G.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, July 2008, p. 1076-1079, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00025-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Toxoplasma gondii Immunoglobulin G (IgG) and IgM Assays Incorporating the New Vidia Analyzer System{triangledown}

Adriana Calderaro,* Giovanna Piccolo, Simona Peruzzi, Chiara Gorrini, Carlo Chezzi, and Giuseppe Dettori

Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Viale A. Gramsci 14, 43100 Parma, Italy

Received 17 January 2008/ Returned for modification 6 March 2008/ Accepted 8 May 2008

The new Vidia system is a fully automated system based on chemiluminescence and antigen bound to magnetic microparticles, which allows a fast measurement of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM levels. The analytical performances of the Vidia Toxo IgG and IgM assays were compared with those of the automated Vidas, AxSYM, and Liaison Toxo IgG and IgM assays. The comparative evaluation was performed utilizing 204 frozen sera belonging to 166 subjects and 201 fresh sera collected from 198 subjects. For the Vidia Toxo IgG system, the sensitivities were 100% in both the retrospective and prospective studies, and specificities were 98.39% in the retrospective study and 100% in the prospective study, respectively. The sensitivities of the other three Toxo IgG assays were 100%, and the specificities ranged from 96.77% to 100%. For the Vidia Toxo IgM assay, the sensitivity and specificity were 100% in both the retrospective and prospective studies. The overall sensitivities and specificities of the other three Toxo IgM assays ranged from 80% to 100% and from 99.44% to 100%, respectively. In our study, the Vidia system revealed excellent sensitivity (100% for both IgG and IgM assays) and good specificity (99.25% for IgG and 100% for IgM assays).

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Viale A. Gramsci 14, 43100 Parma, Italy. Phone: 39 0521 988885. Fax: 39 0521 993620. E-mail: adriana.calderaro{at}unipr.it

{triangledown} Published ahead of print on 14 May 2008.

Clinical and Vaccine Immunology, July 2008, p. 1076-1079, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00025-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»