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Clinical and Vaccine Immunology, June 2008, p. 1031-1033, Vol. 15, No. 6
1071-412X/08/$08.00+0     doi:10.1128/CVI.00424-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Seven Tests for Diagnosis of Human Brucellosis in an Area Where the Disease Is Endemic {triangledown}

M. Concepción Gómez,1* José A. Nieto,2 Carmen Rosa,2 Paloma Geijo,2 M. Ángeles Escribano,3 Amparo Muñoz,3 and Clara López3

Microbiology Laboratory Hospital Severo Ochoa (Leganés), Madrid,1 Department of Internal Medicine,2 Microbiology Laboratory, Hospital Virgen de la Luz, Cuenca, Spain3

Received 27 October 2007/ Returned for modification 4 December 2007/ Accepted 17 April 2008

The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Severo Ochoa, Avda. de Orellana s/n, 28911 Leganés, Madrid, Spain. Phone: 34 91 4818441. Fax: 34 914818442. E-mail: mcongocri{at}hotmail.com

{triangledown} Published ahead of print on 30 April 2008.

Clinical and Vaccine Immunology, June 2008, p. 1031-1033, Vol. 15, No. 6
1071-412X/08/$08.00+0     doi:10.1128/CVI.00424-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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