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Clinical and Vaccine Immunology, May 2008, p. 799-804, Vol. 15, No. 5
1071-412X/08/$08.00+0     doi:10.1128/CVI.00036-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Vaccine-Induced Opsonophagocytic Immunity to Neisseria meningitidis Group B{triangledown}

J. S. Plested and D. M. Granoff*

Center for Immunobiology and Vaccine Development, Children's Hospital Oakland Research Institute, Oakland, California 94609

Received 29 January 2008/ Returned for modification 21 February 2008/ Accepted 12 March 2008

The role of opsonophagocytosis (OP) in protection against meningococcal disease is controversial because patients with deficiencies in terminal complement proteins whose sera support OP but not bactericidal activity (BA) are at greatly increased risk of disease. We assayed complement-mediated BA and OP bactericidal activity in sera from 32 adults immunized with an outer membrane vesicle vaccine given alone or combined with an investigational recombinant protein, genome-derived neisserial antigen (GNA2132). The sera were heat inactivated to remove internal complement activity, and BA was measured with exogenous nonimmune human serum as a complement source. OP was measured with human polymorphonuclear cells (PMNs) and C6-depleted complement, which without PMNs did not support BA. Before immunization, 9 to 19% of sera from subjects in both vaccine groups combined had BA titers of ≥1:4, which increased to 41 to 72% after immunization (P < 0.01 against each of three test strains). The percentages of sera with OP titers of ≥1:5 were 3 to 16%, which increased to 55 to 72% (P < 0.001 for each strain). Most postimmunization BA-positive sera were OP positive, but 10 to 37% of BA-negative sera also were OP positive. Comparing the two vaccine groups, there were no significant differences in the percentages of sera with BA or OP activity except for a higher percentage of OP against one strain in postimmunization sera from subjects in the combination vaccine group (P ≤ 0.02). The data support independent roles for serum BA and OP bactericidal activity in protection against group B disease.

* Corresponding author. Mailing address: 5700 Martin Luther King Jr. Way, Oakland, CA 94609. Phone: (510) 450-7640. Fax: (510) 450-7915. E-mail: dgranoff{at}chori.org

{triangledown} Published ahead of print on 19 March 2008.

Clinical and Vaccine Immunology, May 2008, p. 799-804, Vol. 15, No. 5
1071-412X/08/$08.00+0     doi:10.1128/CVI.00036-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Plested, J. S., Welsch, J. A., Granoff, D. M. (2009). Ex Vivo Model of Meningococcal Bacteremia Using Human Blood for Measuring Vaccine-Induced Serum Passive Protective Activity. CVI 16: 785-791 [Abstract] [Full Text]  
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