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Clinical and Vaccine Immunology, April 2008, p. 726-728, Vol. 15, No. 4
1071-412X/08/$08.00+0     doi:10.1128/CVI.00493-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Histoplasma capsulatum Antigenuria by Ultrafiltration of Samples with False-Negative Results{triangledown}

Lindsey Egan,1* Patricia A. Connolly,1 Deanna Fuller,2 Thomas E. Davis,2 John Witt III,1 Kenneth S. Knox,3 Chadi A. Hage,3 and L. Joseph Wheat1

MiraVista Diagnostics, Indianapolis, Indiana,1 Indiana University School of Medicine, Department of Pathology, Wishard Memorial Hospital, 1001 West Tenth Street, Indianapolis, Indiana 46202,2 Indiana University School of Medicine, Department of Pulmonary Medicine, Roudebush Veterans’ Administration Hospital, 1481 West Tenth Street, Indianapolis, Indiana 462023

Received 18 December 2007/ Returned for modification 14 January 2008/ Accepted 21 February 2008

Patients with histoplasmosis may test falsely negative for Histoplasma capsulatum antigenuria. In some cases antigen is present at levels below the assay's detection limit, and ultrafiltration could improve sensitivity. Antigen was detected following ultrafiltration in 73.8% of falsely negative specimens versus 2% of controls. Ultrafiltration improved sensitivity with a small reduction in specificity.

* Corresponding author. Mailing address: MiraVista Diagnostics, 4444 Decatur Blvd., Suite 300, Indianapolis, IN 46241. Phone: (317) 856-2681. Fax: (317) 856-3685. E-mail: legan{at}miravistalabs.com

{triangledown} Published ahead of print on 27 February 2008.

Clinical and Vaccine Immunology, April 2008, p. 726-728, Vol. 15, No. 4
1071-412X/08/$08.00+0     doi:10.1128/CVI.00493-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Scheel, C. M., Samayoa, B., Herrera, A., Lindsley, M. D., Benjamin, L., Reed, Y., Hart, J., Lima, S., Rivera, B. E., Raxcaco, G., Chiller, T., Arathoon, E., Gomez, B. L. (2009). Development and Evaluation of an Enzyme-Linked Immunosorbent Assay To Detect Histoplasma capsulatum Antigenuria in Immunocompromised Patients. CVI 16: 852-858 [Abstract] [Full Text]  
  • Swartzentruber, S., LeMonte, A., Witt, J., Fuller, D., Davis, T., Hage, C., Connolly, P., Durkin, M., Wheat, L. J. (2009). Improved Detection of Histoplasma Antigenemia following Dissociation of Immune Complexes. CVI 16: 320-322 [Abstract] [Full Text]  


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